In Vitro Antibacterial Activity of Peppertree (Schinus terebinthifolius) Tincture on the Decontamination of Toothbrushes Contaminated with S. mutans

Diana Gabriela de Sousa SOARES, Cibele Braga de OLIVEIRA, Cinira LEAL, Milana Ramos Santana DRUMOND, Wilton Wilney Nascimento PADILHA

Abstract


Objective: To evaluate in vitro the antibacterial activity of 20% Peppertree bark tincture against Streptococcus mutans, and to observe the efficacy of this phytotherapic agent on the decontamination of toothbrushes contaminated with this microorganism. Method: The in vitro antibacterial activity of the tincture against a reference strain of S. mutans (ATCC 2575) was verified for determination of the maximum inhibitory dilution (MID) using the agar diffusion technique. Petri dishes containing Agar Mueller Hinton broth were seeded according to the flooding technique. Seven 6-mm-diameter wells were prepared and inoculated with 50 µL of Peppertree bark tincture in its pure (1:0) and diluted (1:1 to 1:32) forms. 0.12% chlorhexidine solution was used as a positive control. Thereafter, toothbrush decontamination was evaluated in vitro. The toothbrushes were contaminated in BHI broth containing S. mutans by immersing the toothbrush bristles for 1 min. Decontamination was performed by spraying the toothbrushes with the following solutions: Peppertree bark tincture, 0.12% chlorhexidine solution (positive control) and distilled water (negative control). To verify growth inhibition, the occurrence of turbidity of the broth was evaluated after 24-hour incubation in microaerophilia. Seeding was done at 10-3 concentration of Mitis salivarius bacitracin culture medium for cfu/mL count. Results: Data referring to the antibacterial activity against S. mutans showed action of Peppertree tincture up to 1:8 dilution and chlorhexidine up to 1:32 dilution. Regarding toothbrush decontamination, 0.12% chlorhexidine was the only inoculum that remained totally clear after 24 hours. Chlorhexidine presented 0 cfu/mL, Peppertree tincture presented 77 x 10-3 cfu/mL and the negative control presented 4432 x 10-3 cfu/mL. Conclusion: 20% peppertree tincture presented in vitro antibacterial activity against S. mutans and was effective in decreasing the contamination of toothbrushes contaminated with this microorganism.

Keywords


Descontaminação; Fitoterapia; Odontologia preventiva; Decontamination; Phytotherapy; Preventive dentistry.



DOI: http://dx.doi.org/10.4034/pboci.v7i3.171

PBOCI IS A MEMBER OF CROSSREF AND ALL THE CONTENT OF ITS JOURNALS ARE LINKED BY DOIS THROUGH CROSSREF.