In Vitro Evaluation of Fluoride in Saliva After Topical Application of Professional Use Products

Natanael Victor Furtunato Bezerra, Mariana Leonel Martins, Karla Lorene França Leite, Mariana Marinho Davino de Medeiros, Leopoldina de Fátima Dantas de Almeida, Wilton Wilney Nascimento Padilha, Yuri Wanderley Cavalcanti


Objective:To evaluate in vitro the presence of fluoride in saliva after applying professional use products on the surface of dental enamel. Material and Methods:Experimental groups were composed by: Cariostatic 12% (CA), Fluoridated Varnish 5% (FV), Fluorine Acidulate Gel 1.23% (AG) and Fluorine Neutral Gel 2% (NG). Fluoridated dentifrice (FD) and Artificial Saliva (AS) were used as controls. Products (10 μL) were applied to the surface of bovine enamel blocks (4×4×1 mm, n = 18) and immersed in 10 mL of artificial saliva at room temperature. Aliquots of artificial saliva (750 μL) of each sample were collected 1, 2, 4, 8, 24 and 48 hours after application of the products. Analyses were performed in triplicate, using a fluoride ion-specific electrode coupled to a potentiometer. The equipment was calibrated using a standard curve for fluoride analysis from 0.125 to 64 μgF-/mL. Results:Greater fluoride concentration (μgF-/mL) was observed after 1h application, as follows: 197.40 (NG), 172.21 (AG), 20.25 (CA), 14.49 (FV) e 11.81 (FD). Fluoride concentration increased overtime for all groups. After 48h, the following fluoride concentrations were assessed: 428.12 (AG), 267.25 (NG), 65.36 (FV), and 62.52 (CA). Conclusion:Greater fluoride release was observed for AG and NG groups, mostly after 1h application.


Fluoride; Cariostatic Agents; Artificial Saliva; In Vitro Techniques.

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