The Ability of Biodentine™ of Guided Tissue Remineralization (GTR): Analysis Using SEM, EDX and TEM

Endang Suprastiwi, Aditya Wisnu Putranto, Ike Dwi Maharti


Objective:To analyze the Biodentine™ capability in guided tissue remineralization. Material and Methods:Four premolar with two cavities per tooth of 3 mm depth were demineralized with EDTA 17% in shaking incubator at 37°C temperature. After 7 days, the sample were washed with aquabidest then were soaked in 20 ml NaCl 1 M (pH 7.0) at 25°C temperature for 8 hours. The samples were divided into two groups: G1: The control group (cavity directly restored with composite resin); G2: Biodentine™group (cavity with Biodentine™as a base then restored with composite resin). All samples were stored in shaking incubator under PBS solution at 37°C temperature. SEM, EDX and TEM analysis were performed on the 7thand 14th day. Results:The 14thday Biodentine group had the best SEM remineralization feature with irregular dentine tubular features covered by density of mass. In the EDX analysis, the concentration of calcium ion of the Biodentine group was higher than the control group on the 7th day analysis (Biodentin™ 10.2167 and control 1.9667) and on the 14thday analysis (Biodentine™ 29.833 and Control 22.080). The Biodentine™ group and control group of the 7th and 14th day experienced significant increases in calcium ion concentration while the concentration of phosphate ion in the Biodentine™ and control group had a much lower value of calcium either on the 7thor 14th day. The TEM analysis of Biodentine™ group showed more intrafibrillar remineralization than the control group. The feature of intrafibrillar dentin remineralization is analyzed by looking at the density of black dots in collagen. Conclusion:Biodentine™ is able to trigger the process of remineralization by guided tissue remineralization.


Tooth Remineralization; Dentin; Calcium Phosphates.

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Dai L, Liu Y, Salameh Z, Khan S, Mao J, Pashley DH, Tayd FR. Can caries-affected dentin be completely remineralized by guided tissue remineralization? Dent Hypotheses 2011; 2(2):74-82.

Cao CY, Mei ML, Li QL, Lo ECM, Chu CH. Methods for biomimetic remineralization of human dentine: A systematic review. Int J Mol Sci 2015; 16(3):4615-27.

Chen Z, Cao S, Wang H, Li Y, Kishen A, Deng X, et al. Biomimetic remineralization of demineralized dentine using scaffold of CMC/ACP nanocomplexes in an in vitro tooth model of deep caries. PLoS One 2015; 10(1):e0116553.

Vaseenon S. Relationship between caries-affected dentin mineral density and microtensile bond strength. [Thesis]. University of Iowa, 2011. 115pp. Available at: Iowa Research Online: [Accessed on August 15, 2018]

Uskoković V, Bertassoni LE2. Nanotechnology in dental sciences: Moving towards a finer way of doing dentistry. Materials 2010; 3(3):1674-91.

Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Marshall Jr. GW. Biomechanical perspective on the remineralization of dentin. Caries Res 2009; 43(1):70-7.

Garchitorena Ferreira MI. Bioactive materials in dentin remineralization. Odontoestomatologia. 2016; 18(28):11-8.

Priyalakshmi S, Manish R. Review on Biodentine - A bioactive dentine substitute. J Dent Med Sci 2014; 13(1):13-7.

Malkondu O, Kazandağ MK, Kazazoğlu E. A review on Biodentine, a contemporary dentine replacement and repair material. BioMed Res Int 2014; 2014:Article ID 160951.

Tanomaru M, Viapina R, Guerreiro J. From MTA to new biomaterials based on calcium silicate. Odovtos Int J Dent Sci 2016; 18(1):18-22.

Laurent P, Camps J, About I. Biodentine™ induces TGF-g1 release from human pulp cells and early dental pulp mineralization. Int Endod J 2012; 45(5):439-48.

Luo Z, Dongmei L, Kohli M, Yu Q, Kim S, He W. Effect of biodentine on the proliferation, migration and adhesion of human dental pulp stem cells. J Dent 2014; 42(4):490-7.

Septodont Scientific Files. Biodentine active biosilicate technology, 2016. Available at: [Accessed on August 15, 2018]

Rajan N, Habermehl J, Coté MF, Doillon CJ, Mantovani D. Preparation of ready- to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. Nat Protoc 2006; 1(6):2753-8.

Marques MRC, Loebenberg R, Almukainzi M. Simulated biological fluids with possible application in dissolution testing. Dissolution Tech 2011; 15-28.

Scelza MF, Teixeira AM, Scelza P. Decalcifying effect of EDTA-T, 10 % citric acid, and 17 % EDTA on root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003; 95(2):234-6.

Abou Neel EA, Aljabo A, Strange A, Ibrahim S, Coathup M, Young AM, Bozec L, Mudera V. Demineralization – remineralization dynamics in teeth and bone. Int J Nanomedicine 2016; 11:4743-63.

Singh H, Kaur M, Markan S, Kapoor P. Biodentine: A promising dentin substitute. J Interdiscipl Med Dent Sci 2014; 2:140. 2376-032X.1000140