Peculiarities of Morphology of Large Salivary Glands in Experimental Animals Under Different Conditions of Motor Activity of Their Mothers During Pregnancy

Yuliia Slynko, Irina Ivanovna Sokolova, Halyna Gubina-Vakulik, Anatoly Potapchyk, Iryna Korneyko

Abstract


Objective:To evaluate of the microstructure of the salivary glands of experimental animals born from of females rats, who were subjected to different modes of motor activity during the pregnancy: limited and compensated by regular moderate training. Material and Methods:The sample was composed 84 three-month-old descendants of Wistar rats which were divided into three groups: G1 (n = 27): posterity of female rats, who during gestation were in standard conditions; G2 (n = 25): posterity of female rats, who during pregnancy were in hypokinesia conditions; G3 (n = 32): which stayed in conditions of hypokinesia, but had running load in running wheel regularly 5 times a week. Results:In adult descendants of G2, morphological structure of the submandibular salivary glands was characterized by decrease size and number of glandular cells; reduction of their cytoplasm; increase in heterochromatin amount and decrease in the intensity of fuchsinophilia. Microstructure of the submandibular salivary glands of the offspring of G3 indicates positive effect of regular short-term physical activity on the morphofunctional state of the specified glands. An increased number of tissue basophils in their stroma is also in favor of greater functional activity of salivary glands. Conclusion:This study indicates deceleration of the metabolic processes in salivary glands of animals under the influence of hypokinesia of their mothers during pregnancy. It was also confirmed that regular physical activity completely eliminates the negative effect of mothers' hypokinesia on morphology the salivary glands of the descendant.


Keywords


Animal Experimentation; Salivary Glands; Morphological and Microscopic Findings.

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References


Afanasyev VV, Yaglova NV, Khubutiya BN, Krasnikova TV, Zoryan YeV, Khripunkov VA. Morfofunktsionalnyye izmeneniya malykh slyunnykh zhelez u bolnykh s razlichnymi formami sialadenoza. Rossiyskiy stomatologicheskiy Zhurnal 2012; 5:7-10. [In Ukrainian]

Shipsky AV. Xerostomia, hyposalivation and disorders of the excretory evacuation function of the salivary glands (review). Parodontology 2002; 3:45-50.

Carpenter GH. The secretion, components and properties of saliva. Annu Rev Food Sci Technol 2013; 4:267-76. https://doi.org/10.1146/annurev-food-030212-182700

Woof JV, Kerr MA. The function of immunoglobulin A in immunity. J Pathol 2006; 208(2):270-82. https://doi.org/10.1002/path.1877

Soares RV, Lin T, Siqueira CC, Bruno LS, Li X. Salivary micelles identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycosylated proline-rich protein and lysozyme. Arch Oral Biol 2004; 49(5):337-43. https://doi.org/10.1016/j.archoralbio.2003.11.007

Ebersole JL, Dawson D 3rd, Emecen-Huja P, Nagarajan R, Howard K, Grady ME, et al. The periodontal war: microbes and immunity. Periodontol 2000 2017; 75(1):52-115. https://doi.org/10.1111/prd.12222

Leito TD, Ligtenberg AJM, Nazmi K. Identification of salivaty components that include transition of hyphae to yeast in Candida albicans. FEMS Yeast Res 2009; 9(7):1102-10. https://doi.org/10.1111/j.1567-1364.2009.00575.x

Vavilova TP, Shtrunova LN, Shishkin SV, Shishkin VS. The use of mixed saliva laboratory values for evaluation of periodontal tissue condition. Russian J Dent 2010; 1:10-12.

Sichkoriz HA, Minko LY. Changes in physical and chemical properties of oral fluid in patients with periodontal diseases in the settings of chronic hepatitis C during antiviral therapy. Ukrainian Dental Almanach 2016; 3(1):13-7.

Tymofieiev O, Viesova A. Secretion function of big and small salivary glands after the operation on exterpation parotid gland. Modern Dent 2011; 4(58):66-9. [In Ukrainian]

Tymofieiev O. Secretory function of large and small salivary glands at galvanism and galvanosis. Modern Dent 2013; 3(67):72-6. [In Ukrainian]

Antonova LK. Influence of hypokinesia on the state of health of teenage girls. Ross Med Zhurn 2003; 3:35-7.

Kachelayeva YV. Hypodynamia and human health. In the world of scientific discoveries 2010; 4(14):26-7.

Cavill N, Sonja Kahlmeier S, Racioppi F. Physical Activity and Health in Europe: Evidence for Action. - Copenhagen: World Health Organization, 2006. 55p.

Reedeker N, Van Der Mast RC, Giltay EJ, Van Duijn E, Roos RA. Hypokinesia in Huntington's disease co-occurs with cognitive and global dysfunctioning. Mov Disord 2010; 25(11):1612-8. https://doi.org/10.1002/mds.23136

Gubina-Vaculic G, Slynko Y, Sokolova I, Colousova N. Morphofunсtional peculiaritіes of submandibular salivary gland of rats in conditions of experimental hypokinesia in their mothers’ organisms. World Med Biol 2013; 2:26-8.

Kozhemiakin YM, Khromov OS, Filonenko MA, Sayfetdinova HA. Scientific and practical recommendations on the maintenance and handling of laboratory animals. Kyiv: Avicenna, 2002. 156p.

Kozak HI, Zapryvoda LP, Ostapenko OV, Stechenko LO, et al. Histology. Short Course: Textbook. Chaikovskyi YB (Editor). Vinnytsia: New Book, 2016. 336p.




DOI: http://dx.doi.org/10.4034/PBOCI.2019.191.41

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