An in Vitro Evaluation of Remineralizing Capacity of Self-Assembling Peptide (SAP) P11-4 and Casein Phosphopeptides-Amorphous Calcium Phosphate (CPP-ACP) on Artificial Enamel

Prashant Babaji, Mahesh Melkundi, Pooja Bhagwat, Vanisha Mehta


Objective:To determine and compare the remineralizing capacity of self-assembling peptide (SAP) P11-4 and casein phosphopeptides‑amorphous calcium phosphate (CPP-ACP) on enamel. Material and Methods:Enamel samples were divided into 2 groups. Group I was treated with Self‑assembling peptide (SAP) P11-4 and group II with casein phosphopeptides-amorphous calcium phosphate (CPP-ACP). In both groups, remineralizing capacity was assessed at baseline, 2 weeks, 6 weeks and 12 weeks. Student’s t- test and ANOVA were applied, with the significance level set at 5%. Results:The mean calcium weight % was evaluated at baseline, 2 weeks, 6 weeks and 12 weeks. In Group I, there was increase in mean value (62.12 ± 1.24) from baseline to 12 weeks (67.36 ± 2.14). However, there was decrease in phosphate weight % from 37.16 ± 2.52 at baseline to 35.72 ± 2.11 at 12 weeks. In Group II, mean calcium weight % was 64.18 ± 1.52 at baseline, which ultimately increased to 66.01 ± 2.03 at 12 weeks. Phosphate weight % showed reduction from 37.34 ± 2.23 at baseline to 35.04 ± 2.02 at 12 weeks. Ca/P ratio showed significant improvement. There was significant difference in Ca/P ratio at 2 weeks, 6 weeks and 12 weeks in both groups (p<0.05). Conclusion:Self-assembling peptide (SAP) P11-4 found to be more effective and efficient as compared to casein phosphopeptides‑amorphous calcium phosphate (CPP-ACP).


Caseins; Phosphopeptides; Calcium Phosphates; Dental Enamel.

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