Antibacterial Effects of Cuminum cyminum Extract Against Enterococcus Faecalis Biofilms From Clinical Isolates

Rizky Amalia, Siti Utami Dewi, Anggraini Margono, Munyati Usman

Abstract


Objective:To compare the antibacterial efficacy of Cuminum cyminum (cumin) extract and 2% chlorhexidine. Material and Methods:E. faecaliswas isolated from non-vital teeth with chronic apical abscess. Samples were then bred in the ChromAgar medium. Subsequently, E. faecalisbacteria’s DNA extraction was performed. DNA was then amplified by conventional PCR, and the product was run on an electrophoresis gel. Subsequently, we extracted Cuminumcyminumseeds using the steam distillation technique. The extract was diluted at various concentrations: 0.2, 0.5, 0.7, 1.0, and 1.2 mg/mL.The extract’s antibacterial effect was evaluated using an ELISA reader with optical density. Specifically, we assessed the turbidity of E. faecalisin biofilms following immersion in antibacterial agents. Results:In the clinically isolated E. faecalisgroup, the OD values of 0.7 and 1.0 mg/mL cumin extracts were significantly different from that of 0.2 mg/mL cumin extract. A significant difference was also observed between the OD values of 1.0 mg/mL cumin extract and 2% CHX (p<0.05). Conclusion:The antibacterial effect of 1.0 mg/mL Cuminum cyminumextract had higher efficiency than 2% chlorhexidine against E. faecalisbiofilms from clinical isolates.

Keywords


Plants, Medicinal; Biofilms; Chlorhexidine; Enterococcus faecalis.

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References


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