Protein Profiles of Individuals with Erosive Tooth Wear

Aida Mulic, Anne Bjorg Tveit, Nicholas Modesto Vieira, Kirsten Limesand, Alexandre Rezende Vieira


Objective: To determine if protein profiles identified in saliva could be used to determine risk and severity of erosive tooth wear. Material and Methods:Three types of saliva sampling were performed to obtain saliva from 34 18-year old individuals that received regular dental check-ups, along with clinical status of the dentition and risk factor related to erosive tooth wear using the VEDE scale. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample to test for association with erosive tooth wear status. Results: There were no differences in the number of detectable proteins sourced from the parotid gland, nor the unstimulated and stimulated whole saliva. Five out of the 34 individuals had no signs of erosive tooth wear despite an acidic diet and were more likely to have proteins with molecular weight smaller than 1 KDa (p=0.03). Conclusion: There is potential for the use of protein profiling to determine risks for erosive tooth wear.


Dental Enamel; Dental Caries; Tooth Erosion.

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Schlueter N, Amaechi BT, Bartlett D, Buzalaf MAR, Carvalho TS, Ganss C, et al. Terminology of erosive tooth wear: Consensus report of a workshop organized by ORCA and the Cariology Research Group of the IADR. Caries Res 2020; 54(1):2-6.

Hove LH, Stenhagen KR, Mulic A, Holme B, Tveit AB. May caries-preventive fluoride regimes have an effect on dental erosive wear? An in situ study. Acta Odontol Scand 2015; 73(2):114-20.

Vieira AR, Chung C, Raffensperger SK, Muluk P. Milk reverts the effects of an enamel erosive but healthy diet. Pesq Bras Odontopediatria Clín Integrada 2018; 18(1):e3848.

Pineda ÁEGA, Borges-Yáñez SA, Irigoyen-Camacho ME, Lussi A. Relationship between erosive tooth wear and beverage consumption among a group of schoolchildren in Mexico City. Clin Oral Invest 2019; 23(2):715-23.

Cassiano LPS, Ventura TMS, Silva CMS, Leite AL, Magalhāes AC, Pessan JP, et al. Protein profile of the acquired enamel pellicle after rinsing with whole milk, fat-free milk, and water: an in vivo study. Caries Res 2018; 52(4):288-96.

Vieira AR, Modesto A, Marazita ML. Caries: Review of human genetics research. Caries Res 2014; 48(5):491-506.

Søvik JB, Vieira AR, Tveit AB, Mulic A. Enamel formation genes associated with dental erosive wear. Caries Res 2015; 49(3):236-42.

Uhlen MM, Stenhagen KR, Dizak PM, Holme B, Mulic A, Tveit AB, et al. Genetic variation may explain why females are less susceptible to dental erosion. Eur J Oral Sci 2016; 124(5):426-32.

Pappa E, Vastardis H, Mermelekas G, Gerasimidi-Vazeou A, Zoidakis J, Vougas K. Saliva proteomics analysis offers insights on type 1 diabetes pathology in a pediatric population. Front Physiol 2018; 9:444.

Zhao M, Yang Y, Guo Z, Shao C, Sun H, Zhang Y, et al. A comparative proteomics analysis of five body fluids: Plasma, urine, cerebrospinal fluid, amniotic fluid and saliva. Proteomics Clin Appl 2018; 12(6):e1800008.

Mulic A, Tveit AB, Skaare AB. Prevalence and severity of dental erosive wear among a group of Norwegian 18-year olds. Acta Odontol Scand 2013; 71(3-4):475-81.

Mulic A, Tveit AB, Wang NJ, Hove LH, Espelid I, Skaare AB. Reliability of two clinical scoring systems for dental erosive wear. Caries Res 2010; 44(3):294-9.

Statistics Norway. Income. Statistisk sentralbyrå. 2011. Available from: [Accessed on January 28, 2020].

Statistics Norway. Immigration and immigrants. Statistisk sentalbyrå. 2010. Available from: [Accessed on January 28, 2020].

Grundmann O, Fillinger JL, Victory KR, Burd R, Limesand KH. Restoration of radiation therapy-induced salivary gland dysfunction in mice by post therapy IGF1 administration. BMC Cancer 2010; 10:417.

Perinpanayagam HE, Van Wuyckhuyse BC, Ji ZS, Tabak LA. Characterization of low-molecular-weight peptides in human parotid saliva. J Dent Res 1995; 74(1):345-50.

Salas MMS, Nascimento GG, Huysmans MC, Demarco FF. Estimated prevalence of erosive tooth wear in permanent teeth of children and adolescents: an epidemiological systematic review and meta-regression analysis. J Dent 2015; 43(1):42-50.

Uhlen MM, Mulic A, Holme B, Tveit AB, Stenhagen KR. The susceptibility to dental erosion differs among individuals. Caries Res 2016; 50(2):117-23.

Vieira AR. On the genetics contribution to molar incisor hypomineralization. Int J Paediatr Dent 2019; 29(1):2-3.