Contamination of Resin Composites in Dental Practice

Camila FERRAZ, Cleiryanne ROCHA, Márcia Maria Negreiros Pinto ROCHA, Maria da Glória Almeida MARTINS, Paula JACQUES

Abstract


Objective: To verify the presence or absence of microorganisms in resin composite samples (RCS), photoactivated or not, and on the surfaces of RC cartridges (RCC) during clinical use in three dental offices (A, B, C) at the city of Fortaleza, CE, Brazil.
Method: The following groups were formed in the three dental offices, according to the substrates analyzed for contamination: G1 (control, n=10) - non-photoactivated RCS, G2 (n=10) - RCS photoactivated with LED, G3 (n=10) - RCS photoactivated with halogen light, G4 (n=15) - RCC after clinical use. In the dental offices B and C, an additional G5 (n=5, control) was composed of RCC disinfected with alcohol 70 after clinical use. A total of 145 specimens were obtained. Swabs were used for sample collection in G4 and G5. In G1 to G3, 2-mm-thick increments of RC were obtained and photoactivated or not for 20 seconds with a LED or halogen light source. The samples were then placed in test tubes with BHI broth and maintained under refrigeration until incubation in microaerophilia.
Results: Contamination was found in 46.9% of the samples; G4 and G5 had 100% of contaminated samples (mean McFarland value of 7.0 ± 3.02). There was no statistically significant difference in the frequency of contamination among the dental offices A, B and C, in all groups, after analysis by the chi-square and Kruskal-Wallis tests (p=0.367 and p=0.090). There was no statistically significant difference between G1 and G2 and G3 after analysis by the Mann-Whitney test (p=0.396) nor between G2 and G3 (p=0.487) after analysis by the Pearson’s chi-square test. Cocci (isolated or arranged in pairs, chains or clusters), bacilli (isolated or arranged in pairs or chains) and yeast were identified.
Conclusion: Microorganisms were detected on the RC samples and on the surfaces of the RC cartridges in the three dental offices evaluated in this study.

Keywords


Resinas compostas; Contaminação; Bactérias; Microbiologia; Composite resins; Contamination; Bacteria; Microbiology.



DOI: http://dx.doi.org/10.4034/pboci.v10i1.813

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