@article{Pedrosa_Alves_Rahhal_Nogueira_Sipert_2022, title={Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells}, volume={22}, url={https://revista.uepb.edu.br/PBOCI/article/view/954}, abstractNote={<div><strong><span lang="EN-US">Objective: </span></strong><span lang="EN-US">To </span><span lang="EN-US">compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs)</span><span lang="EN-US">. </span><strong><span lang="EN-US">Material and Methods: </span></strong><span lang="EN-US">The culture of hPDLSCs was established. Cell density was set at 2 × 10<sup>4</sup> cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey’s test (</span><span lang="EN-US">α</span><span lang="EN-US">=0.05). </span><strong><span lang="EN-US">Results: </span></strong><span lang="EN-US">At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control</span><span lang="EN-US">. </span><strong><span lang="EN-US">Conclusion: </span></strong><span lang="EN-US">Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.</span></div>}, journal={Pesquisa Brasileira em Odontopediatria e Clínica Integrada}, author={Pedrosa, Marlus da Silva and Alves, Tomaz and Rahhal, Juliana Garuba and Nogueira, Fernando Neves and Sipert, Carla Renata}, year={2022}, month={Jan.}, pages={e210114} }