TY - JOUR AU - Rodrigues, Gabriella Fernandes AU - Monteiro, Amanda Souza Nunes AU - Vollú, Ana Lúcia AU - Pires, Paula Maciel AU - Sá Silva, Aline Saddock de AU - Lopes, Ricardo Tadeu AU - Oliveira, Branca Heloisa AU - Neves, Aline de Almeida AU - Barja-Fidalgo, Fernanda AU - Fonseca-Gonçalves, Andréa PY - 2022/03/16 Y2 - 2024/03/29 TI - Efficacy of 30% and 38% Silver Diamine Fluoride in Arresting Caries Lesions After Different Application Times: An in Vitro Study JF - Pesquisa Brasileira em Odontopediatria e Clínica Integrada JA - Pesqui Bras Odontopediatria Clin Integr VL - 22 IS - SE - DO - UR - https://revista.uepb.edu.br/PBOCI/article/view/1046 SP - e210112 AB - <div><strong><span lang="EN-US">Objective:&nbsp;</span></strong><span lang="EN-US">To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times</span><span lang="EN-US">.&nbsp;</span><strong><span lang="EN-US">Material and Methods:&nbsp;</span></strong><span lang="EN-US">Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3).&nbsp;</span><strong><span lang="EN-US">Results:&nbsp;</span></strong><span lang="EN-US">Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth</span><span lang="EN-US">.&nbsp;</span><strong><span lang="EN-US">Conclusion:&nbsp;</span></strong><span lang="EN-US">The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal.</span></div> ER -