TY - JOUR AU - Kashi, Faezeh AU - Abbasi, Mahdi AU - Ahmadi, Elham AU - Omrani, Ladan Ranjbar AU - Rafeiee, Niyousha AU - Kharazifard, Mohammad Javad PY - 2022/09/20 Y2 - 2024/03/29 TI - Cytotoxicity Comparison of a Calcium Silicate-Based Resin Cement versus Conventional Self-Adhesive Resin Cement and a Resin-Modified Glass Ionomer: Cell Viability Analysis JF - Pesquisa Brasileira em Odontopediatria e ClĂ­nica Integrada JA - Pesqui Bras Odontopediatria Clin Integr VL - 22 IS - SE - DO - UR - https://revista.uepb.edu.br/PBOCI/article/view/1465 SP - e210185 AB - <div><strong><span lang="EN-US">Objective:&nbsp;</span></strong><span lang="EN-US">To compare the cytotoxicity level of a new calcium silicate-based resin cement (TheraCem) with two commonly used cements, including a conventional self-adhesive resin cement (Panavia SA) and a resin-modified glass ionomer cement (FujiCem2), on the human gingival fibroblast cells after 24 and 48 hours</span><span lang="EN-US">.&nbsp;</span><strong><span lang="EN-US">Material and Methods:&nbsp;</span></strong><span lang="EN-US">Twelve discs of each cement type were fabricated. The extract of cement disks was made by incubating them in the cell medium. Human gingival fibroblast cells were cultured and exposed to cement extracts for 24 h and 48 h. MTT assay was performed on extracts and optical density and cell viability rates were calculated by the spectrophotometer device at 570 nm. Data were analyzed using ANOVA and Tukey HSD tests.&nbsp;</span><strong><span lang="EN-US">Results:&nbsp;</span></strong><span lang="EN-US">The cell viability rates after 24 hours and 48 hours were as follows: TheraCem: 89.24% and 85.46%, Panavia SA: 49.51% and 46.57% and FujiCem2: 50.63% and 47.36%. TheraCem represented the highest cell viability rate. However, no significant difference was noted between Panavia SA and FujiCem2. Time had no significant effect on cell viability</span><span lang="EN-US">.&nbsp;</span><strong><span lang="EN-US">Conclusion:&nbsp;</span></strong><span lang="EN-US">TheraCem exhibited the best results among three tested cements and was considered non-toxic. Panavia SA and FujiCem2 were not significantly different regarding the cell viability rate. Time had no significant effect on the cytotoxicity level of cements.</span></div> ER -