Antimicrobial activity of Xylosma prockia (Turcz.) Turcz. leaves against Candida parapsilosis in vitro.

Authors

  • Guilherme Fernando da Silva
  • Queila da Silva Rosa
  • Mariany Lopes da Costa Folly
  • Gustavo José Cota Freitas
  • Daniel de Assis Santos
  • Karen Luise Lang
  • Gabriella Freitas Ferreira UFJF

Abstract

Candida parapsilosis is one of the most common pathogens causing candidemia in Brazil. Available therapies to treat candidiasis are limited due to high toxicity and high fungal resistance to clinically available drugs. Natural products are a promising source for the discovery and development of new antimicrobial agents. In this way, we aimed to investigate the antifungal activity of Xylosma prockia (Turcz.) Turcz. leaves against C. parapsilosis ATCC 22019 in vitro. The minimum inhibitory concentrations of the ethanolic extract and the fractions were determined. The fraction with the best activity was selected for further experiments: lipid peroxidation, ergosterol content and reactive species production analyses. The ethanolic extract, butanol, dichloromethane and ethyl acetate fractions inhibited fungal growth at concentrations below 100 mg/L. The ethyl acetate fraction inhibited the growth cell at the lowest concentration (16 mg/L). The time-kill curve test showed that this fraction has a fungicidal effect. The mechanism of action did not appear to be related to steroid synthesis, as the fraction did not affect ergosterol content. Interestingly, the fraction induced the oxidative and nitrosative burst that causes lipid peroxidation. The results of this study demonstrate that the caffeoylquinic acids, caffeoylglycosides and coumaroylglycosides present in the ethyl acetate fraction of X. prockia leaves have antifungal potential due to oxidative damage.

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Published

01/02/2025

How to Cite

da Silva, G. F., da Silva Rosa, Q., Lopes da Costa Folly, M., Cota Freitas, G. J., de Assis Santos, D., Lang, K. L., & Freitas Ferreira, G. (2025). Antimicrobial activity of Xylosma prockia (Turcz.) Turcz. leaves against Candida parapsilosis in vitro. BIOFARM - Journal of Biology & Pharmacy and Agricultural Management, 19(1), 25–33. Retrieved from https://revista.uepb.edu.br/BIOFARM/article/view/4097

Issue

Vol. 19 No. 1 (2024): Revista BIOFARM- Fluxo Contínuo

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Articles

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