Comparison of Oncostatin M in Patients with Chronic Periodontitis with and without Diabetes

Amulya  Kolluri; Pratibha  Gopalkrishna; Venkat Rao  Josyula; Aditya Kiran  Gatta; Kalyana Pentapati  Chakravarthy

Authors

Amulya Kolluri
Pratibha Gopalkrishna
Venkat Rao Josyula
Aditya Kiran Gatta
Kalyana Pentapati Chakravarthy

Keywords:

Diabetes Mellitus, Periodontitis, Gingiva, Inflammation, Cytokines

Abstract

Objective: To compare the Oncostatin M (OSM) concentrations in tissues of patients with chronic periodontitis with and without diabetes. Material and Methods: Sixty-four subjects visiting the dental outpatient department were categorized as “healthy” (Group 1), “periodontitis” (Group 2), and “diabetes with periodontitis” (Group 3) groups. The clinical oral examination included assessment of plaque, gingivitis, probing depth, clinical attachment level. Blood glucose was assessed for group 3 patients. OSM concentration in the tissues was assessed using ELISA in all groups. Results: The mean OSM was 0.02 ± 0.04 pg/mg in the healthy group, 0.12 ± 0.09 pg/mg in the chronic periodontitis group and 0.13 ± 0.10 pg/mg in the diabetes-periodontitis group. A significantly higher mean OSM was seen in Group 2 and Group 3 than Group 1. The amount of OSM positively correlated with probing depth and clinical attachment level. Conclusion: Periodontal disease causes a rise in Oncostatin M, independent of the diabetic status. Expression of OSM in the gingival tissues can serve as an inflammatory marker.

References

Verhulst MJL, Loos BG, Gerdes VEA, Teeuw WJ. Evaluating all potential oral complications of diabetes mellitus. Front Endocrinol 2019; 10:56. https://doi.org/10.3389/fendo.2019.00056

Lalla E, Lamster IB, Drury S, Fu C, Schmidt AM. Hyperglycemia, glycoxidation and receptor for advanced glycation endproducts: Potential mechanisms underlying diabetic complications, including diabetes-associated periodontitis. Periodontol 2000 2000; 23:50-62. https://doi.org/10.1034/j.1600-0757.2000.2230104.x

Perraudin JP. The biochemical aspects of diabetes in oral health. Br J Diabetes 2019; 19(2):93-8. https://doi.org/https://doi.org/10.15277/bjd.2019.222

Houben E, Hellings N, Broux B. Oncostatin M: an underestimated player in the central nervous system. Front Immunol 2019; 10:1165. https://doi.org/10.3389/fimmu.2019.01165

Goyden J, Tawara K, Hedeen D, Willey JS, Oxford JT, Jorcyk CL. The effect of OSM on MC3T3-E1 osteoblastic cells in simulated microgravity with radiation. PLoS One 2015; 10(6):e0127230. https://doi.org/10.1371/journal.pone.0127230

Modur V, Feldhaus MJ, Weyrich AS, Jicha DL, Prescott SM, Zimmerman GA, et al. Oncostatin M is a proinflammatory mediator. In vivo effects correlate with endothelial cell expression of inflammatory cytokines and adhesion molecules. J Clin Invest 1997; 100(1):158-68. https://doi.org/10.1172/JCI119508

Garcia JP, Utomo L, Rudnik-Jansen I, Du J, Zuithoff NPA, Krouwels A, et al. Association between Oncostatin M expression and inflammatory phenotype in experimental arthritis models and osteoarthritis patients. Cells 2021; 10(3):508. https://doi.org/10.3390/cells10030508

West NR, Hegazy AN, Owens BMJ, Bullers SJ, Linggi B, Buonocore S, et al. Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor–neutralizing therapy in patients with inflammatory bowel disease. Nat Med 2017; 23(5):579-89. https://doi.org/10.1038/nm.4307

Stawski L, Trojanowska M. Oncostatin M and its role in fibrosis. Connect Tissue Res 2019; 60(1):40-9. https://doi.org/10.1080/03008207.2018.1500558

Akarsu M, Hurşitoğlu M, Toprak Z, Yoldemir ŞA, Altun Ö, Toprak ID, et al. Relationships among oncostatin M, insulin resistance, and chronic inflammation: a pilot study. Arch Endocrinol Metab 2019; 64(1):38-44. https://doi.org/10.20945/2359-3997000000176

Pradeep AR, Thorat Manojkumar S, Garima G, Raju A. Serum levels of oncostatin M (a gp 130 cytokine): an inflammatory biomarker in periodontal disease. Biomarkers 2010; 15(3):277-82. https://doi.org/10.3109/13547500903573209

Thorat M, AR P, Garg G. Correlation of levels of Oncostatin M cytokine in crevicular fluid and serum in periodontal disease. Int J Oral Sci 2010; 2(4):198-207. https://doi.org/10.4248/IJOS10077

Khan F, Rathod S, Kolte A, Gupta M, Chari S, Gonde N. Quantitative analysis of oncostatin M levels in chronic periodontitis patients. J Int Clin Dent Res Organ 2020; 12(1):33-37. https://doi.org/10.4103/jicdro.jicdro_51_19

Jones MM, Vanyo ST, Ibraheem W, Maddi A, Visser MB. Treponema denticola stimulates Oncostatin M cytokine release and de novo synthesis in neutrophils and macrophages. J Leukoc Biol 2020; 108(5):1527-41. https://doi.org/10.1002/JLB.4MA0620-072RR

Löe H, Silness J. Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontol Scand 1963; 21:533-51. https://doi.org/10.3109/00016356309011240

Silness J, Löe H. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964; 22:121-35. https://doi.org/10.3109/00016356408993968

Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Matsuo T. Oncostatin M synergistically induces CXCL10 and ICAM-1 expression in IL-1β-stimulated-human gingival fibroblasts. J Cell Biochem 2010; 111(1):40-8. https://doi.org/10.1002/jcb.22648

Lin SJ, Chen YL, Kuo MY Bin, Li CL, Lu HK. Measurement of gp130 cytokines - Oncostatin M and IL-6 in gingival crevicular fluid of patients with chronic periodontitis. Cytokine 2005; 30(4):160-7. https://doi.org/10.1016/j.cyto.2004.12.018

Lu H-K, Chen Y-L, Chang H-C, Li C-L, Kuo MYP. Identification of the osteoprotegerin/receptor activator of nuclear factor-kappa B ligand system in gingival crevicular fluid and tissue of patients with chronic periodontitis. J Periodontal Res 2006; 41(4):354-60. https://doi.org/10.1111/j.1600-0765.2006.00883.x

Zhou L, Bi C, Gao L, An Y, Chen F, Chen F. Macrophage polarization in human gingival tissue in response to periodontal disease. Oral Dis 2019; 25(1):265-73. https://doi.org/10.1111/odi.12983

Hanes PJ, Krishna R. Characteristics of inflammation common to both diabetes and periodontitis: are predictive diagnosis and targeted preventive measures possible? EPMA J 2010; 1(1):101-16.

https://doi.org/10.1007/s13167-010-0016-3