Efficacy of 30% and 38% Silver Diamine Fluoride in Arresting Caries Lesions After Different Application Times: An in Vitro Study

Gabriella Fernandes  Rodrigues; Amanda Souza Nunes  Monteiro; Ana Lúcia  Vollú; Paula Maciel  Pires; Aline Saddock de  Sá Silva; Ricardo Tadeu  Lopes; Branca Heloisa  Oliveira; Aline de Almeida  Neves; Fernanda  Barja-Fidalgo; Andréa  Fonseca-Gonçalves

Authors

Gabriella Fernandes Rodrigues
Amanda Souza Nunes Monteiro
Ana Lúcia Vollú
Paula Maciel Pires
Aline Saddock de Sá Silva
Ricardo Tadeu Lopes
Branca Heloisa Oliveira
Aline de Almeida Neves
Fernanda Barja-Fidalgo
Andréa Fonseca-Gonçalves

Keywords:

Dental Caries, Cariostatic Agents, Dentin, Tooth Remineralization, X-Ray Microtomography

Abstract

Objective: To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times. Material and Methods: Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3). Results: Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth. Conclusion: The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal.

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