Soluble Human Leukocyte Antigen Molecules Detected in Orofacial Cleft Patients: A Case-Control Study

Regina Purnama Dewi  Iskandar; Alida; Ari  Triwardhani; Ida Bagus  Narmada; Faizah  Hanum; Arief Haryo  Kusumo; Chairul Anwar  Nidom; Sri Agus  Sudjarwo

Authors

Regina Purnama Dewi Iskandar
Alida
Ari Triwardhani
Ida Bagus Narmada
Faizah Hanum
Arief Haryo Kusumo
Chairul Anwar Nidom
Sri Agus Sudjarwo

Keywords:

Cleft Palate, Cleft Lip, HLA Antigens

Abstract

Objective: To compare soluble HLA-C and HLA-DR molecules present in the plasma of orofacial cleft and non-orofacial cleft populations. Material and Methods: Orofacial cleft patients were recruited using an accidental sampling approach (n=15). Peripheral blood was collected from the participants and processed for Enzyme Linked Immunosorbent Assay (ELISA) against HLA-C and HLA-DR with specific antibodies. The absorbance was calculated utilizing ELISA reader. Data were statistically analyzed using an independent t-test to compare the disease and control groups. Results: The levels of soluble HLA-C and HLA-DR were significantly higher in the diseased group compared to the control group (p<0.05). Conclusion: The role of HLA molecules in non-communicable disease and congenital anomalies, particularly orofacial cleft, remains speculative despite the positive results of this study and those of previous investigations. It suggests that the variables examined may affect specific pathways involved in the pathogenesis of orofacial cleft, and predispose the individuals concerned to the oral cleft.

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