Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis


  • Pablo Alejandro Millones Gómez
  • Margarita Fe Requena Mendizábal
  • Roger Damaso Calla Poma
  • Tania Valentina Rosales Cifuentes
  • Federico Martin Malpartida Quispe
  • Dora Jesús Maurtua Torres
  • Reyma Evelyn Bacilio Amaranto
  • Carlos Alberto Minchón Medina
  • Lusin Antonio Ponce Contreras


Psidium, Biofilms, Gram-Negative Bacteria


Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitroMaterial and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii(p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.


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How to Cite

Gómez, P. A. M. ., Mendizábal, M. F. R. ., Poma, R. D. C. ., Cifuentes, T. V. R. ., Quispe, F. M. M. ., Torres, D. J. M. ., Amaranto, R. E. B. ., Medina, C. A. M. ., & Contreras, L. A. P. . (2022). Antibacterial and Antiadhesion Effects of Psidium guajava Fractions on a Multispecies Biofilm Associated with Periodontitis. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 22, e210080. Retrieved from



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