Effect of Xylitol Varnishes on the Inhibition of Demineralization in Vitro


  • Paula Andery Naves
  • Alexandre Lima de Moura
  • Marcela Charantola Rodrigues
  • Michele Baffi Diniz
  • Victor Ellias Arana-Chavez
  • Maísa Camillo Jordão
  • Cristiane de Almeida Baldini Cardoso


Dental Caries, Tooth Demineralization, Xylitol


Objective: To evaluate the efficacy of xylitol varnishes in the inhibition of enamel demineralization in vitroMaterial and Methods: Bovine enamel blocks (n=120) were randomly allocated to four groups (n = 30), and the surface hardness (SH) was measured at baseline. The blocks were treated with the following varnishes: 20% xylitol, 20% xylitol plus F (5% NaF), Duraphat™ (5% NaF, positive control), and placebo (no-F/xylitol, negative control). The varnishes were applied and removed after 6 h of immersion in artificial saliva. The blocks were subjected to pH cycles (demineralization and remineralization for 2 and 22h/day, respectively, for 8 days). Surface and cross-sectional hardnesses were measured to calculate the percentage of SH loss (%SHL) and the integrated loss of the subsurface hardness (ΔKHN). Data were statistically analyzed using Kruskal-Wallis and Tukey’s tests (p<0.05). Results: %SHL was significantly decreased by 20% xylitol plus F, Duraphat™, and 20% xylitol varnishes compared to placebo. The use of 20% xylitol plus F varnish led to a significantly lower percentage of SH loss compared to the use of 20% xylitol varnish without F. However, the experimental and commercial varnishes led to significantly lower subsurface demineralization compared to placebo and did not differ from each other. Conclusion: Xylitol varnishes, especially when combined with F, effectively prevent enamel demineralization.


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How to Cite

Naves, P. A. ., de Moura, A. L. ., Rodrigues, M. C. ., Diniz, M. B. ., Arana-Chavez, V. E. ., Jordão, M. C., & Cardoso, C. de A. B. . (2022). Effect of Xylitol Varnishes on the Inhibition of Demineralization in Vitro. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 22, e210205. Retrieved from https://revista.uepb.edu.br/PBOCI/article/view/1451



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