Association between Volatile Sulfur Compounds Prevotella intermedia and Matrix Metalloproteinase-8 Expression


  • Fergy Christin Maitimu
  • Yuniarti Soeroso
  • Hari Sunarto
  • Boy Muchlis Bachtiar


Halitosis, Periodontitis, Prevotella intermedia, Matrix Metalloproteinase-8


Objective: To determine the correlation between levels of methyl mercaptan (CH3SH) hydrogen sulfide (H2S), the proportion of Prevotella intermedia (Pi), and matrix metalloproteinase-8 (MMP-8) gene expression levels in periodontitis patients accompanied by halitosis. Material and Methods: Samples were obtained from gingival crevicular fluid (GCF) in the deepest pocket and by swabbing in the tongue coating area in patients with periodontitis presenting with halitosis (n = 23) and healthy subjects as controls (n = 7). The values of CH3SH and H2S were obtained using Oral Chroma. The proportion of Pi and MMP-8 expression levels were evaluated using PCR-RT. All the result was statistically analyzed using SPSS software. Results: The levels of CH3SH and H2S in participants with PD ≥ 6 mm showed a robust negative correlation with the proportion of P. intermedia in GCF and tongue coating. No statistically significant association was detected between CH3SH and H2S levels and MMP-8 expression levels (p>0.05). Conclusion:  There is no association between CH3SH and H2S levels, the proportion of P. intermedia, and MMP-8 expression in patients with periodontitis accompanied by halitosis.


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How to Cite

Maitimu, F. C. ., Soeroso, Y. ., Sunarto, H. ., & Bachtiar, B. M. . (2022). Association between Volatile Sulfur Compounds Prevotella intermedia and Matrix Metalloproteinase-8 Expression. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 22, e210002. Retrieved from



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