Obliteration of Dentinal Tubules by Desensitizing Agents Based on Silver Fluoride/Potassium Iodide or Pre-Reacted Glass Particles: An in Vitro Study


  • Fernanda Novais Arantes Maciel de Castro
  • Diandra Costa Arantes
  • Wagner Nunes Rodrigues
  • Allyson Nogueira Moreira
  • Cláudia Silami Magalhães


Dentin, Dentin Desensitizing Agents, Dentin Sensitivity, Microscopy, Electron, Scanning


Objective: To evaluate the efficacy of desensitizing agents for the obliteration of dentinal tubules subjected or not to a simulated oral environment. Material and Methods: Dentinal discs (n=8) treated with Riva-Star (RS) or PRG-Barrier-Coat (PRG) were submitted (cycled) or not submitted (control) to erosive-abrasive-thermal cycles and evaluated using scanning electron microscopy/energy dispersive spectroscopic analysis. The variables analyzed were tubule obliteration and dentin surface chemical composition. Data were analyzed by non-parametric tests (p<0.05). Results: The cycled and control groups did not differ significantly for the responses in each material. The PRG control and cycled groups had fewer visible tubules and a higher proportion of totally obliterated tubules than the RS groups. The percentages of silver coverage were higher in the RS-control than in the RS-cycled. There was a significant inverse correlation between the presence of silver and non-obliterated tubules (R=-0.791; p<0.001). The percentages of carbon, aluminum, strontium, and potassium were significantly higher in the PRG-control and PRG-cycled compared to the RS control. The percentages of calcium, phosphorus, and silver were significantly higher in the RS compared to the PRG groups. PRG-control showed a higher percentage of boron than RS-control. Conclusion: PRG promoted greater tubule obliteration than SR. Simulated stress did not affect the obliterating effect of each agent. Greater silver coverage corresponded to a lower proportion of non-obliterated tubules in RS. Carbon, aluminum, strontium, boron, and potassium predominated in the dentin surface treated with PRG, while calcium, phosphorus, and silver prevailed in RS groups.


Zeola LF, Soares PV, Cunha-Cruz J. Prevalence of dentin hypersensitivity: systematic review and meta-analysis. J Dent 2019; 81:1-6. https://doi.org/10.1016/j.jdent.2018.12.015

Douglas-De-Oliveira DW, Vitor GP, Silveira JO, Martins CC, Costa FO, Cota LOM. Effect of dentin hypersensitivity treatment on oral health related quality of life - a systematic review and meta-analysis. J Dent 2018; 71:1-8. https://doi.org/10.1016/j.jdent.2017.12.007

Jena A, Shashiekha G. Comparison of efficacy of three different desensitizing agents for in-office relief of dentin hypersensitivity: a 4 weeks clinical study. J Conserv Dent 2015; 18(5):389-95. https://doi.org/10.4103/0972-0707.164052

Majji P, Murthy KRV. Clinical efficacy of four interventions in the reduction of dentinal hypersensitivity: a 2-month study. J Dent Res 2016; 27(5):477-82. https://doi.org/10.4103/0970-9290.195618

Brännström M, Astrom A. A study on the mechanism of pain elicited by dentin. J Dent Res 1964; 43:619-25. https://doi.org/10.1177/00220345640430041601

Moraschini V, Da Costa LS, Dos Santos GO. Effectiveness for dentin hypersensitivity treatment of non-carious cervical lesions: a meta-analysis. Clin Oral Investig 2018; 22(2):617-31. https://doi.org/10.1007/s00784-017-2330-9

Chen CL, Parolia A, Pau A, Porto ICCM. Comparative evaluation of the effectiveness of desensitizing agentes in dentine tubule occlusion using scanning electron microscopy. Aust Dent J 2015; 60(1):65-72. https://doi.org/10.1111/adj.12275

Wang Z, Jiang T, Sauro S, Pashley DH, Toledano M, Osorio R, et al. The dentine remineralization activity of a desensitizing bioactive glass-containing toothpaste: an in vitro study. Aust Dent J 2011; 56(4):372-81. https://doi.org/10.1111/j.1834-7819.2011.01361.x

Willershausen I, Schulte D, Azaripour A, Weyer V, Briseño B, Willershausen B. Penetration potential of a silver diamine fluoride solution on dentin surfaces. An ex vivo study. Clin Lab 2015; 61(11):1695-1701. https://doi.org/10.7754/clin.lab.2015.150401

Craig GG, Knight GM, Mcintyre JM. Clinical evaluation of diamine silver fluoride/potassium iodide as a dentine desensitizing agent. A pilot study. Aust Dent J 2012; 57(3):308-11. https://doi.org/10.1111/j.1834-7819.2012.01700.x

Greenhill JD, Pashley DH. The effects of desensitizing agents on the hydraulic conductance of human dentin in vitro. J Dent Res 1981; 60(3):686-98. https://doi.org/10.1177/00220345810600030401

Arita S, Suzuki M, Kazama-Koide M, Shinkai K. Shear bond strengths of tooth coating materials including the experimental materials contained various amounts of multi-ion releasing fillers and their effects for preventing dentin demineralization. Odontology 2017; 105(4):426-36. https://doi.org/10.1007/s10266-016-0290-1

Fujimoto Y, Iwasa M, Murayama R, Miyazaki M, Nagafuji A, Nakatsuka T. Detection of ions released from S-PRG fillers and their modulation effect. Dent Mater J 2010; 29(4):92-7. https://doi.org/10.4012/dmj.2010-015

Ikemura K, Tay FR, Endo T, Pashley DH. A review of chemical-approach and ultramorphological studies on the development of fluoride-releasing dental adhesives comprising new pre-reacted glass ionomer (PRG) fillers. Dent Mater J 2008; 27(3):315-39. https://doi.org/10.4012/dmj.27.315

Ito S, Iijima M, Hashimoto M, Tsukamoto N, Mizoguchi I, Saito T. Effects of surface pre-reacted glass-ionomer fillers on mineral induction by phosphoprotein. J Dent 2011; 39(1):72-9. https://doi.org/10.1016/j.jdent.2010.10.011

Murayama R, Furuichi T, Yokokawa M, Takahashi F, Kawamoto R, Takamizawa T, et al. Ultrasonic investigation of the effect of S-PRG filler-containing coating material on bovine tooth demineralization. Dent Mater J 2012; 31(6):954-9. https://doi.org/10.4012/dmj.2012-153

Ma S, Imazato S, Chen JH, Mayanagi G, Takahashi N, Ishimoto T, et al. Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces. Dent Mater J 2012; 31(6):909-915. https://doi.org/10.4012/dmj.2012-061

Schmalz G, Hellwig F, Mausberg RF, Schneider H, Krause F, Haak R, et al. Dentin protection of different desensitizing varnishes during stress simulation: an in vitro study. Oper Dent 2017; 42(1):E35-E43. https://doi.org/10.2341/16-068-L

Han L, Okamoto A, Fukushima M, Okiji T. Evaluation of a new fluoride-releasing one-step adhesive. Dent Mater J 2006; 25(3):509-15. https://doi.org/10.4012/dmj.25.509

Saori M, Saori T, Matsuda Y, Naoki H, Sano H, Masamitsu K. Caries prevention after surface reaction-type prereacted glass ionomer filler-containing coating resin removal from root surfaces. J Nanosci Nanotechnol 2016; 16(12):12996-13000. https://doi.org/10.1166/jnn.2016.13653

Selvaraj K, Sampath V, Sujatha V, Mahalaxmi S. Evaluation of microshear bond strength and nanoleakage of etch-and-rinse and self-etch adhesives to dentin pretreated with silver diamine fluoride/potassium iodide: An in vitro study. Indian J Dent Res 2016; 27(4):421-5. https://doi.org/10.4103/0970-9290.191893

Mei ML, Ito L, Cao Y, Li QL, Lo EC, Chu CH. Inhibitory effect of silver diamine fluoride on dentine demineralisation and collagen degradation. J Dent 2013; 41(9):809-17. https://doi.org/10.1016/j.jdent.2013.06.009

Cai J, Burrow MF, Manton DJ, Tsuda Y, Sobh EG, Palamara JEA. Effects of silver diamine fluoride/potassium iodide on artificial root caries lesions with adjunctive application of proanthocyanidin. Acta Biomater 2019; 88:491-502. https://doi.org/10.1016/j.actbio.2019.02.020

Machado AC, Rabelo FEM, Maximiano V, Lopes RM, Aranha ACC, Scaramucci T. Effect of in-office desensitizers containing calcium and phosphate on dentin permeability and tubule occlusion. J Dent 2019; 86:53-9. https://doi.org/10.1016/j.jdent.2019.05.025




How to Cite

de Castro, F. N. A. M., Arantes, D. C., Rodrigues, W. N., Moreira, A. N., & Magalhães, C. S. (2022). Obliteration of Dentinal Tubules by Desensitizing Agents Based on Silver Fluoride/Potassium Iodide or Pre-Reacted Glass Particles: An in Vitro Study. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 22, e210183. Retrieved from https://revista.uepb.edu.br/PBOCI/article/view/1629



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