Accelerating Wound Healing of Traumatic Ulcer with Topical Application of Dental Pulp Mesenchymal Stem Cell Secretome and Robusta Green Coffee Bean Extract Combination in vivo

Ericko Ichi; Iin Eliana Triwahyuni; Desi Sandra Sari

Authors

Ericko Ichi
Iin Eliana Triwahyuni
Desi Sandra Sari

Keywords:

Fibroblasts, Neutrophils, Stem Cells, Wound Healing, Medicine

Abstract

Objective: To investigate the ability of a combination dental pulp mesenchymal stem cell secretome (DPMSCS), robusta green coffee bean extract (RGCBE), and Carboxymethylcellulose-Natrium (CMC-Na) in a Wistar rats model of traumatic ulcers. Material and Methods: Twenty-eight young, male, healthy Wistar rats (Rattus norvegicus) were divided into seven groups randomly: Group K₀, group K₁-3 (traumatic ulcer rats that received CMC-Na gel for three days), group K₁-7 (traumatic ulcer rats that received CMC-Na gel for seven days), group K₂-3 (traumatic ulcer rats that received RGCBE for three days), group K₂-7 (traumatic ulcer rats that received RGCBE for seven days), group K₃-3 (traumatic ulcer rats that received DPMSCS for three days), and group K₃-7 (traumatic ulcer rats that received DPMSCS for seven days). An ulcer was made with an amalgam stopper on the right buccal mucosa of the rats. DPMSCS 50% gel was applied to the ulcer on the left buccal mucosa. The ulcer diameter was measured on day 3 and day 7. Results: There was a significant difference in the diameter of the ulcer, the number of neutrophils, and fibroblasts in the treatment group compared to the control group on day 7. Conclusion: A combination of DPMSCS and RGCBE 50% accelerates traumatic ulcer wound healing by lowering ulcer diameter, decreasing neutrophil counts, and increasing fibroblast proliferation in vivo.

References

WHO. Burns. Fact Sheets 2018. Available from: https://www.who.int/en/news-room/fact-sheets/detail/burns. [Accessed on June 19, 2019].

Zhang P, Zou B, Liou Y-C, Huang C. The pathogenesis and diagnosis of sepsis post burn injury. Burn Trauma 2021; 9:tkaa047. https://doi.org/10.1093/burnst/tkaa047

Forbinake NA, Ohandza CS, Fai KN, Agbor VN, Asonglefac BK, Aroke D, et al. Mortality analysis of burns in a developing country: a CAMEROONIAN experience. BMC Public Health 2020; 20:1269. https://doi.org/10.1186/s12889-020-09372-3

Regezi JA, Verstraete FJM. Clinical-Pathologic Correlations. In: Verstraete FJM, Lommer MJ. Oral and Maxillofacial Surgery in Dogs and Cats. W.B. Saunders; 2012. https://doi.org/10.1016/B978-0-7020-4618-6.00039-7

Surboyo MDC, Arundina I, Rahayu RP. Increase of collagen in diabetes-related traumatic ulcers after. Dental Journal (Majalah Kedokteran Gigi) 2017; 50(2):71-5. https://doi.org/10.20473/j.djmkg.v50.i2.p71-75

Apriasari ML, Rahayu RP, Ernawati DS. Mauli banana stem extract application increased expression of NF-kB in traumatic ulcer healing. Dental Journal 2018; 51(2):67-70. https://doi.org/10.20473/j.djmkg.v51.i2.p67-70

Srivastava R, Kundu A, Pradhan D, Jyoti B, Chokotiya H, Parashar P. A comparative study to evaluate the efficacy of Curcumin Lozenges (TurmNova®) and intralesional corticosteroids with hyaluronidase in management of oral submucous fibrosis. J Contemp Dent Pract 2021; 22(7):751-5. https://doi.org/10.5005/jp-journals-10024-3144

Muttaqin Z, Lina Hadi, Zara Maghfirah. Efficacy of Robusta coffee bean extract (Coffea robusta) against bacterial growth of Staphylococcus aureus. Biosci Med J Biomed Transl Res 2022; 6:1675-9. https://doi.org/10.37275/bsm.v6i4.493

Nugraha A, Purwati, Susilowati H, Hendrianto E, Karsari D, Ertanti N, et al. Medicinal Signaling Cells Metabolite Oral Based as a Potential Biocompatible Biomaterial Accelerating Oral Ulcer Healing (In Vitro Study). Eur Dent J 2019; 13(3):432-6. https://doi.org/10.1055/s-0039-1693923

Sunarharum WB, Yuwono SS, Aziza OF. Study on the effect of roasting temperature on antioxidant activity of early-roasted Java coffee powder (Arabica and Robusta). IOP Conf Ser Earth Environ Sci 2019; 230:012045. https://doi.org/10.1088/1755-1315/230/1/012045

Farah A. Nutritional and health effects of coffee. In: Lashermes P. Achieving sustainable cultivation of coffee. Breeding and quality traits. Burleigh Dodds Science Publishing, Cambridge, UK, 2018. 259-90. https://doi.org/10.19103/as.2017.0022.14

Tajik N, Tajik M, Mack I, Enck P. The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. Eur J Nutr 2017; 56(7):2215-44. https://doi.org/10.1007/s00394-017-1379-1

Rezkia F, Wibawa K, Nugraha A. Curcumin loaded chitosan nanoparticle for accelerating the post extraction wound healing in diabetes mellitus patient: a review. Res J Pharm Technol 2020; 13(2):1039-42. https://doi.org/10.5958/0974-360X.2020.00191.2

Suciadi S, Nugraha A, Ernawati D, Ayuningtyas N, Narmada I, Prahasanti C, et al. The efficacy of human dental pulp stem cells in regenerating submandibular gland defects in diabetic wistar rats (Rattus novergicus). Res J Pharm Tech 2019; 12(4):1573-9. https://doi.org/10.5958/0974-360X.2019.00261.0

Sarasati A, Rezkita F, Wibawa K, Winias S, Nugraha AP, Ridwan RD, et al. Gingival mesenchymal stem cells, concentrated growth factors and silk-fibroin scaffold to alleviate peripheral nerve regeneration: a review. Biochem Cell Arch 2020; 20(1 Suppl):2921-9. https://doi.org/10.35124/bca.2020.20.S1.2921

Aprilianti N, Rahmadhani D, Rizqianti Y, Ridwan RD, Ramadhani N, Nugraha A. Periodontal ligament stem cells, solcoseryl pasta incoporated nano-hydroxyapatite silica gel scaffold for bone defect regeneration in chronic periodontitis: A review. Biochem Cell Arch 2020(1 Suppl); 20:3101-6. https://doi.org/10.35124/bca.2020.20.S1.3101

Sari DS, Bumi MEC. Potensi Asam Klorogenik Dari Biji Kopi Robusta Sebagai Stimulator Sel Punca Mesensimal Pulpa Gigi Dalam Meningkatkan Kecepatan Migrasi, Proliferasi Dan Kemampuan Diferensiasi Osteogenik Sebagai Strategi Terapi Regenerasi Jaringan Periodontal. Lap Penelit Hibah IDB Intern UNEJ 2019. [In Indonesian].

Hernawati S, Zikra YA, Fatmawati DWA. The effects of topical application of red pomegranate (Punica granatum Linn) extract gel on the healing process of traumatic ulcers in Wistar rats. Dent J (Majalah Kedokt Gigi) 2019; 52(2):90-4. https://doi.org/10.20473/j.djmkg.v52.i2.p90-94

Tigner A, Ibrahim SA, Ian M. Histology, White Blood Cell. Blood Cell. 2021 Nov 19. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022.

Dick MK, Miao JH, Limaiem F. Histology, Fibroblast. 2022 May 8. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022.

Hendriati L, Hamid IS, Widodo T, Wandasari C, Risata PM. Effect of Egg White Gel againts Burn Healing on White Rat (Rattus novergicus). J Ilmu Kefarmasian Indones 2018; 16(2):231. https://doi.org/10.35814/jifi.v16i2.532

Serafini S, Santos MM, Aoun Tannuri AC, Zerbini MCN, de Mendonça Coelho MC, de Oliveira Gonçalves J, et al. Is hematoxylin-eosin staining in rectal mucosal and submucosal biopsies still useful for the diagnosis of Hirschsprung disease? Diagn Pathol 2017; 12(1):84. https://doi.org/10.1186/s13000-017-0673-9

Pramono A, Adiwinarno B, Mayasari LO. Efektivitas pemberian ekstrak gel Belimbing Manis (Averrhoa carambola Linn) terhadap kesembuhan ulkus traumatikus studi in vivo terhadap mukosa tikus (Strain Wistar ). Pros Semin Nas Publ Hasil-Hasil Penelit Dan Pengabdi Masy “Implementasi Penelit Dan Pengabdi Masy Untuk Peningkatan Kekayaan Intelekt 2017; Universita: 39-44. [In Indonesian].

Groeger S, Meyle J. Oral mucosal epithelial cells. Front Immunol 2019; 10:208. https://doi.org/10.3389/fimmu.2019.00208

Sari DS, Maduratna E, Bumi C, Sudiana I-K, Rantam FA. Role of secretome mesenchymal stem cells as immunomodulator in periodontal diseases. J Dentomaxillofacial Sci 2021; 6(3):139-46. https://doi.org/10.15562/jdmfs.v6i3.989

Rahmawati D, Nugraha AP, Ardani IGAW, Triwardhani A, Narmada IB. Role of hematopoetic stem cell in inflammatory response during orthodontic tooth movement: a narrative review. Biochem Cell Arch 2020; 20(1):2879-82. https://doi.org/10.35124/bca.2020.20.S1.2879

Arimbi D, Yuwono HS. pH of wound fluids treated using coffee powder and bacitracin-neomycin powder. Glob j Surg 2016; 4(1):9-11. https://doi.org/10.12691/js-4-1-3

Xia J, Minamino S, Kuwabara K, Arai S. Stem cell secretome as a new booster for regenerative medicine. Biosci Trends 2019; 13(4):299-307. https://doi.org/10.5582/bst.2019.01226

Shiddiqi T, Adnan Z, Nurudhin A. Pengaruh secretome sel punca mesenkimal terhadap ekspresi interleukin-1β Dan Caspase-1. Biomedika 2018; 9(2):31-8. https://doi.org/10.23917/biomedika.v9i2.5840 [In Indonesian].

Martin M, Sari D, Mantika R, Praharani D. Combination of dental pulp stem-cell secretome and Robusta coffee bean extract (Coffea canephora) in enhancing osteocalcin and alkaline phosphatase expression in periodontitis-induced wistar rats. J Orofac Sci 2021; 13(2):136-41. https://doi.org/10.4103/jofs.jofs_157_21

Kenisa YP, Istiati I, Juliastuti WS. Effect of Robusta coffee beans ointment on full thickness wound healing. Dent J (Majalah Kedokt Gigi) 2012; 45(1):52-57. https://doi.org/10.20473/j.djmkg.v45.i1.p52-57

Fatimatuzzahro N, Ermawati T, Prasetya RC, Destianingrum PQ. Efek pemberian gel ekstrak biji kopi robusta (Coffea canephora) terhadap jumlah osteoblas dan osteoklas pada tulang alveolar tikus periodontitis. Padjadjaran J Dent Res Students 2020; 4(2):128-133. https://doi.org/10.24198/pjdrs.v4i2.28383 [In Indonesian].

Narmada IB, Putri PD, Lucynda L, Triwardhani A, Ardani IGAW, Nugraha AP. Effect of caffeic acid phenethyl ester provision on fibroblast growth Factor-2, matrix metalloproteinase-9 expression, osteoclast and osteoblast numbers during experimental tooth movement in Wistar rats (Rattus norvegicus). Eur J Dent 2021; 15(2):295-301. https://doi.org/10.1055/s-0040-1718640

Naveed M, Hejazi V, Abbas M, Kamboh AA, Khan GJ, Shumzaid M, et al. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomed Pharmacother 2018; 97:67-74. https://doi.org/10.1016/j.biopha.2017.10.064

Ojeh N, Stojadinovic O, Pastar I, Sawaya A, Yin N, Tomic-Canic M. The effects of caffeine on wound healing. Int Wound J 2016; 13(5):605-13. https://doi.org/10.1111/iwj.12327

Nugraha AP, Narmada IB, Ernawati DS, Dinaryanti A, Hendrianto E, Riawan W, et al. Bone alkaline phosphatase and osteocalcin expression of rat’s Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study). Eur J Dent 2018; 12(4):566-73. https://doi.org/10.4103/ejd.ejd_261_18

Ma S, Xie N, Li W, Yuan B, Shi Y, Wang Y. Immunobiology of mesenchymal stem cells. Cell Death Differ 2014; 21(2):216-25. https://doi.org/10.1038/cdd.2013.158

Nugraha A, Triyono E, Prahasanti C, Sufiawati I, Prasetyo R, Ernawati D. The correlation of pathognomonic periodontal manifestation with CD4+ level in people live with Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome in a Tertiary Hospital, Surabaya, Indonesia. J Int Oral Heal 2019; 11(3):137-40. https://doi.org/10.4103/jioh.jioh_53_19