Single Nucleotides Polymorphisms in COX2 Gene and their Association with Signs and Symptoms of Teething – A Pilot Study

Alana Gonçalvez Mauta; Caio Luiz Bitencourt Reis; Vinicius Broska Teodoro; Isabela Ribeiro Madalena; Christian Kirschneck; Peter Proff; Aleysson Olimpio Paza; Flares Baratto-Filho; Daniela Silva Barroso de Oliveira; Erika Calvano Küchler; João Armando Brancher

Authors

Alana Gonçalvez Mauta
Caio Luiz Bitencourt Reis
Vinicius Broska Teodoro
Isabela Ribeiro Madalena
Christian Kirschneck
Peter Proff
Aleysson Olimpio Paza
Flares Baratto-Filho
Daniela Silva Barroso de Oliveira
Erika Calvano Küchler
João Armando Brancher

Keywords:

Cyclooxygenase 2, Genes, Tooth Eruption

Abstract

Objective: To investigate the association between single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) and local and systemic signs and symptoms of teething. Material and Methods: Forty-four pairs of mothers-babies/toddlers were included. Erupted primary teeth were evaluated during clinical examination. Local and systemic signs and symptoms of teething were obtained from mothers' reporting via anamnesis. Samples of buccal cells were retrieved for DNA genotyping using real-time PCR. The T-test, Chi-square test, logistic regression, and haplotype analyses were applied. Results: Almost all mothers (95.5%) reported at least one local or systemic sign and symptom of teething. The most common was increased salivation (79.5%), diarrhea (72.3%), and fever (70.5%). The mean number of signs and symptoms per child was higher in boys than girls (mean = 5.1; SD= 1.5; p=0.008). Sleep disturbance (p=0.03) and loss of appetite (p=0.05) were more reported in boys. The rs689466 and rs5275 were not associated with signs and symptoms of teething (p>0.05). Conclusion: The single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) were not associated with local and systemic signs and symptoms of teething.

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