Salivary Metabolites in Patients with Mucopolysaccharidosis


  • Rafaela de Oliveira Torres
  • Andréa Vaz Braga Pintor
  • Tatiana Kelly da Silva Fidalgo
  • Ana Paula Canedo Valente
  • Liana Bastos Freitas-Fernandes
  • Ivete Pomarico Ribeiro de Souza


Mucopolysaccharidoses, Saliva, Magnetic Resonance Spectroscopy, Metabolomics


Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods:The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4ºC, and the supernatants (500µl) were stored at -80ºC until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.


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How to Cite

Torres, R. de O., Pintor, A. V. B., Fidalgo, T. K. da S., Valente, A. P. C., Freitas-Fernandes, L. B., & de Souza, I. P. R. (2023). Salivary Metabolites in Patients with Mucopolysaccharidosis. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 24, e220151. Retrieved from



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