Cross-Sectional Microhardness and Chemical Composition of Primary Teeth with Green Discoloration due to Hyperbilirubinemia
Keywords:
Tooth, Child, Hyperbilirubinemia, Liver Diseases, Hardness TestsAbstract
Objective: To evaluate the chemical components and cross-sectional microhardness of primary teeth with hyperbilirubinemia-induced green pigmentation. Material and Methods: The sample consisted of two anterior and two posterior green primary teeth discolored by bilirubin and regular primary teeth, paired according to child age and tooth type. Scanning energy dispersive spectroscopy was used to investigate the mass percentage of calcium, phosphorus, and carbon, and a microhardness tester was used to assess the cross-sectional microhardness of enamel and dentin. The collected data were analyzed using the Student's t-test and Mann-Whitney (p<0.05). Results: There was a significant decrease in calcium in the dentin of the green discoloration group compared with the control group. Although the differences in cross-sectional microhardness were not significant, all data showed lower microhardness in both enamel and dentin in the green discoloration group. Conclusion: The calcium content in the dentin of green-discolored primary teeth is lower than in regular primary teeth, but the microhardness of enamel and dentin is not affected.
References
Macedo AF, Costa C, Mattar RHGDM, Azevedo RA. Dystrophic calcification of maxillary sinus in pediatric patients with liver transplantation and pigmentation of dental organ. Rev Paul Pediatr 2017; 36(1):4. https://doi.org/10.1590/1984-0462/;2018;36;1;00012
Alanzi A, Alkheder M, Qudeimat M. Oral health status of Kuwaiti children with a history of chronic liver disease. Med Princ Pract 2019; 28(4):341-346. https://doi.org/10.1159/000499594
Chung PHY, Zheng S, Tam PKH. Biliary atresia: East versus west. Semin Pediatr Surg 2020; 29(4):150950. https://doi.org/10.1016/j.sempedsurg.2020.150950
Bonnet AL, Greset V, Davit-Beal T. Oral manifestations of Alagille syndrome. BMJ Case Rep 2020; 13(5):e234689. https://doi.org/10.1136/bcr-2020-234689
Vidigal EA, Abanto J, Haddad AE, Porta G, Alves FA, BÖnecker M. Oral health-related quality of life among pediatric liver transplant candidates. Braz Oral Res 2020; 34:e100. https://doi.org/10.1590/1807-3107bor-2020.vol34.0100
Seremidi K, Kavvadia K, Tosios K, Pandi-Tsami A. Dental intrinsic green pigmentation from primary to mixed dentition: clinical and histological findings. Eur J Paediatr Dent 2017; 18(2):149-152.
Barbério GS, Zingra ACG, Santos PSS, Machado MAAM. Green teeth related to bilirubin levels. Acta Stomatol Croat 2018; 52(1):61-64. https://doi.org/10.15644/asc52/1/9
Lamba G, Nagpal DI, Korishettar R. Green pigmentation of teeth caused by neonatal cholestatic jaundice and sepsis: A case report. Int J Clin Pediatr Dent 2019; 12(6):566-568. https://doi.org/10.5005/jp-journals-10005-1696
do Valle IB, Eduardo VM, Goese TB, Oliveira ZFL, von Zeidler SV. Green teeth in the primary and permanent dentition. J Pediatr 2017; 191:275-275. https://doi.org/10.1016/j.jpeds.2017.08.014
Neves-Silva R, Alves FA, Antunes A, Goes MF, Giannini M, Tenório MD, et al. Decreased dentin tubules density and reduced thickness of peritubular dentin in hyperbilirubinemia-related green teeth. J Clin Exp Dent 2017; 9(5):e622-e628. https://doi.org/10.4317/jced.53490
Sandoval MJ, Zekeridou A, Spyropoulou V, Courvoisier D, Mombelli A, McLin V, et al. Oral health of pediatric liver transplant recipients. Pediatr Transplant 2017; 21(7). https://doi.org/10.1111/petr.13019
da Silva KTL, Grazziotin-Soares R, de Miranda RR, Novais VR, Carvalho EM, da Silva GR, et al. Effect of an enamel matrix derivative (Emdogain) on the microhardness and chemical composition of human root dentin: An in vitro study. Sci Rep 2022; 12(1):8874. https://doi.org/10.1038/s41598-022-13081-9
Syed S, Yassin SM, Almalki AY, Ali SAA, Alqarni AMM, Moadi YM, et al. Structural changes in primary teeth of diabetic children: Composition and ultrastructure analysis. Children 2022; 26;9(3):317. https://doi.org/10.3390/children9030317
Campos PH, Andrade RSL, Rojas PL, Ferreira MCD, Santos MTBR, Guaré RO, et al. Enamel microhardness of primary teeth of cerebral palsy children. Rev Odontol UNESP 2015; 44(1):1-5. https://doi.org/10.1590/1807-2577.1061
Carvalho FS, Feitosa VP, Fonteles CR, Ribeiro TR, Araújo BS, Ayala AP, et al. Compositional and microhardness findings in tooth affected by X-linked hypophosphatemic rickets. J Clin Exp Dent 2020; 12(7):e688-e694. https://doi.org/10.4317/jced.56945
Watanabe K, Shibata T, Kurosawa T, Morisaki I, Kinehara M, Igarashi S, et al. Bilirubin pigmentation of human teeth caused by hyperbilirubinemia. J Oral Pathol Med 1999; 28(3):128-130. https://doi.org/10.1111/j.1600-0714.1999.tb02010.x
Weerakoon AT, Cooper C, Meyers IA, Condon N, Sexton C, Thomson D, et al. Does dentine mineral change with anatomical location, microscopic site and patient age? J Struct Biol X 2022; 23(6):100060. https://doi.org/10.1016/j.yjsbx.2022.100060
Yamaza H, Sonoda S, Nonaka K, Kukita T, Yamaza T. Pamidronate decreases bilirubin-impaired cell death and improves dentinogenic dysfunction of stem cells from human deciduous teeth. Stem Cell Res Ther 2018; 8;9(1):303. https://doi.org/10.1186/s13287-018-1042-7
Vennila V, Madhu V, Rajesh R, Ealla KK, Velidandla SR, Santoshi S. Tetracycline-induced discoloration of deciduous teeth: case series. J Int Oral Health 2014; 6(3):115-119.
Yazdizadeh M, Sharifi M, Torabi Parizi A, Alipour F, Ghasempuor M, Zanguei E, et al. Dental management of a pediatric patient with progressive familial intrahepatic cholestasis having dental anomalies: A case report and brief review of the literature. BMC Oral Health 2023; 23(1):10. https://doi.org/10.1186/s12903-022-02593-5
Davidovich E, Polak D, Brand HS, Shapira J, Shapiro R. Salivary biochemical variables in liver transplanted children and young adults. Eur Arch Paediatr Dent 2021; 22(2):257-263. https://doi.org/10.1007/s40368-020-00573-3
Donassollo TA, Romano AR, Demarco FF, Della-Bona A. Surface microhardness evaluation of enamel and dentin in bovine and human teeth (permanent and deciduous). Rev Odonto Ciênc 2007; 22(58):311-316.
Hadzić N, Davenport M, Tizzard S, Singer J, Howard ER, Mieli-Vergani G. Long-term survival following Kasai portoenterostomy: is chronic liver disease inevitable? J Pediatr Gastroenterol Nutr 2003; 37(4):430-433. https://doi.org/10.1097/00005176-200310000-00006
Chardot C, Carton M, Spire-Bendelac N, Le Pommelet C, Golmard J, Reding R, et al. Is the Kasai operation still indicated in children older than 3 months diagnosed with biliary atresia? J Pediatr 2001; 138(2):224-228. https://doi.org/10.1067/mpd.2001.111276
Karrer FM, Price MR, Bensard DD, Sokol RJ, Narkewicz MR, Smith DJ, et al. Long-term results with the Kasai operation for biliary atresia. Arch Surg 1996; 131(5):493-496. https://doi.org/10.1001/archsurg.1996.01430170039006
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