Tara Gum as a Controlled Delivery System of Fluoride in Toothpaste: In Vitro Enamel Remineralization Study
Keywords:Biomedical and Dental Materials, Polymers, Dentifrices, Toothpastes, Fluoride
AbstractObjective: To evaluate the remineralizing potential of a hydrocolloid-based, controlled fluoride-releasing system added to dentifrice formulas. Material and Methods: Sixty-five human enamel blocks were prepared and the surface microhardness (SH0) values were determined. The artificial caries lesions were induced and the demineralization surface microhardness (SH1) was evaluated. The blocks were randomly allocated into five groups (n = 13): (1) 100-TGF (100% NaF with Tara gum added); (2) 50-TGF (50% free NaF + 50% NaF with Tara gum added); (3) 100% TG (100% Tara gum without fluoride); (4) 100% NaF (positive control); and (5) placebo (without Tara gum and NaF). The blocks were submitted to 7 days pH cycling and treated with dentifrice slurries twice a day. Finally, surface hardness (SH2) was assessed and the percentage of surface hardness recovery (%SMHR) was calculated. Analysis of variance (ANOVA) followed by Bonferroni test was used for statistical analysis. Results: A positive %SMHR was found in the 100% NaF (5.07) and 50-TGF (0.64) groups, while the 100-TGF (-1.38), 100% TG (-3.88) and placebo (-0.52) did not undergo remineralization. Statistically significant differences were observed between 100% NaF and all the groups except for 50-TGF (p<0.05). Conclusion: The presence of hydrocolloid (Tara gum) promoted minimal remineralization when associated with NaF. In the applied model, Tara gum may have compromised remineralization, preventing free fluoride from acting effectively in the carious lesion.
Rølla G, Ogaard B, Cruz RA. Clinical effect and mechanism of cariostatic action of fluoride-containing toothpastes: a review. Int Dent J 1991; 41(3):171-4.
Lippert F. An introduction to toothpaste - Its purpose, history and ingredients. Monogr Oral Sci 2013; 23:1-14. https://doi.org/10.1159/000350456
Cury JA, Tenuta LMA. Evidence-based recommendation on toothpaste use. Braz Oral Res 2014; 28(Spec No):1-7. https://doi.org/10.1590/S1806-83242014.50000001
Damle SG, Bector A, Damle D, Kaur S. Effect of dentifrices on their remineralizing potential in artificial carious lesions: An in situ study. Dent Res J 2016; 13(1):74-9. https://doi.org/10.4103/1735-3327.174721
Duckworth RM, Jones S. On the relationship between the rate of salivary flow and salivary fluoride clearance. Caries Res 2015; 49(2):141-6. https://doi.org/10.1159/000365949
Chen F, Wang D. Novel technologies for the prevention and treatment of dental caries: a patent survey. Expert Opin Ther Pat 2010; 20(5):681-94. https://doi.org/10.1517/13543771003720491
Alves VF, Moreira VG, Soares AF, Albuquerque LS, Moura HS, Silva AO, et al. A randomized triple-blind crossover trial of a hydrocolloid-containing dentifrice as a controlled-release system for fluoride. Clin Oral Investig 2018; 22(9):3071-7. https://doi.org/10.1007/s00784-018-2395-0
Fagioli L, Pavoni L, Logrippo S, Pelucchini C, Rampoldi L, Cespi M, et al. Linear viscoelastic properties of selected polysaccharide gums as function of concentration, pH, and temperature. J Food Sci 2019; 84(1):65-72. https://doi.org/10.1111/1750-3841.14407
Kumar A., Lather A, Kumar V, Vikash, Sherawat R, Tyagi V. Pharmacological potential of plant used in dental care: A review. J Herb Drugs 2015; 5(4):179-86.
Wu Y, Ding W, Jia L, He Q. The rheological properties of tara gum (Caesalpinia spinosa). Food Chem 2015; 168:366-71. https://doi.org/10.1016/j.foodchem.2014.07.083
Prajapati VD, Jani GK, Moradiya NG, Randeria NP. Pharmaceutical applications of various natural gums, mucilages and their modified forms. Carbohydr Polym 2013; 92(2):1685-99. https://doi.org/10.1016/j.carbpol.2012.11.021
Goswami S, Naik S. Natural gums and its pharmaceutical application. J Sci Innov Res 2014; 3(1):112-21.
Queiroz CS, Hara AT, Paes Leme AF, Cury JA. pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization. Braz Dent J 2008; 19(1):21-7. https://doi.org/10.1590/s0103-64402008000100004
Vieira AEM, Delbem ACB, Sassaki KT, Rodrigues E, Cury JA, Cunha RF. Fluoride dose response in pH-cycling models using bovine enamel. Caries Res 2005; 39(6):514-20. https://doi.org/10.1159/000088189
Dimeski G, Badrick T, St John A. Ion selective electrodes (ISEs) and interferences - a review. Clin Chim Acta 2010; 411(5–6):309-17. https://doi.org/10.1016/j.cca.2009.12.005
Buzalaf MAR, Hannas AR, Magalhães AC, Rios D, Honório HM, Delbem ACB. pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. J Appl Oral Sci 2010; 18(4):316-34. https://doi.org/10.1590/s1678-77572010000400002
Dehailan LA, Martinez-Mier EA, Eckert GJ, Lippert F. An in vitro investigation of anticaries efficacy of fluoride varnishes. Oper Dent 2019; 44(5):E234-E243. https://doi.org/10.2341/18-040-L
Gavic L, Gorseta K, Borzabadi-Farahani A, Tadin A, Glavina D. Influence of toothpaste pH on its capacity to prevent enamel demineralization. Contemp Clin Dent 2018; 9(4):554-9. https://doi.org/10.4103/ccd.ccd_667_18
Pinto SCTP, Araújo KC, Barbosa JR, Cancio V, Rocha AA, Tostes MA. Effect of dentifrice containing fTCP, CPP-ACP and fluoride in the prevention of enamel demineralization. Acta Odontol Scand 2018; 76(3):188-94. https://doi.org/10.1080/00016357.2017.1401658
Amaechi BT. Protocols to study dental caries in vitro: pH cycling models. Methods Mol Biol 2019; 1922:379-92. https://doi.org/10.1007/978-1-4939-9012-2_34
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