Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements


  • Nayara Nery de Oliveira Cunha
  • Marina Azevedo Junqueira
  • Leopoldo Cosme-Silva
  • Laís da Silveira Terra Santos
  • George Augusto Veloso de Oliveira
  • Rafael Tobias Moretti Neto
  • Denismar Alves Nogueira
  • Maísa Ribeiro Pereira Lima Brigagão
  • Ana Beatriz da Silveira Moretti


Biocompatible Materials, Dental Pulp, Dental Pulp Capping, Pulpotomy, Peroxidase


Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.


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How to Cite

Cunha, N. N. de O. ., Junqueira, M. A., Cosme-Silva, L. ., Santos, L. da S. T. ., de Oliveira, G. A. V. ., Moretti Neto, R. T. ., Nogueira, D. A. ., Brigagão, M. R. P. L. ., & Moretti, A. B. da S. (2021). Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 21, e0106. Retrieved from



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