Comparison of Transforming Growth Factor 1 (TGF-β1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation
Keywords:Platelet-Rich Fibrin, Cell Differentiation, Dental Pulp, Culture Media
Objective: To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and Methods: hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni’s post hoc test with significant values (p<0.05). Results: Significant differences were noted in TGF-β1 expression between 1%, 5%, 10%, and 25% L-PRF and the control group (10% FBS). The highest TGF-β1 expression occurred with 25% L-PRF (0.645 ± 0.048), followed by 10% L-PRF (0.461 ± 0.035), 10% FBS (0.374 ± 0.013), 5% L-PRF (0.275 ± 0.045), and the lowest expression was with 1% L-PRF (0.160 ± 0.045). Conclusion: The best result of TGF-B1 expression in hDPSC differentiation was in the 25% L-PRF group.
Chaparro O, Linero I. Regenerative Medicine : A New Paradigm in Bone Regeneration. In: Zorzi AR, Miranda JB. Advanced Techniques in Bone Regeneration. London: IntechOpen Limited; 2016. pp. 253-274. https://doi.org/10.5772/62523
Žižka R, Šedý J. Paradigm shift from stem cells to cell-free regenerative endodontic procedures: a critical review. Stem Cells Dev 2017; 26(3):147-53. https://doi.org/10.1089/scd.2016.0264
Backly RM El, Marei MK. Dental Pulp Stem Cells in Tissue Engineering and Regenerative Medicine: Opportunities for Translational Research. In: El-Badri N. Advances in Stem Cell Therapy: Bench to Bedside. Cham: Springer Nature Switzerland AG.; 2017. p. 171-196.
Niwa T, Yamakoshi Y, Yamazaki H, Karakida T, Chiba R, Hu JC-C, et al. The dynamics of TGF-β in dental pulp, odontoblasts and dentin. Sci Rep 2018; 8(1):4450. https://doi.org/10.1038/s41598-018-22823-7
Rosa WLO, Piva E, Silva AF. Disclosing the physiology of dental pulp for vital pulp therapy. Int Endod J 2018; 51(8):829-86. https://doi.org/10.1111/iej.12906
Fujioka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang Y, Choukroun J. Optimized platelet-rich fibrin with the low-speed concept: growth factor release, biocompatibility, and cellular response. J Periodontol 2017; 88(1):112-21. https://doi.org/10.1902/jop.2016.160443
Choukroun J, Aalam AA, Miron RJ. Platelet Rich Fibrin “PRF” and Regenerative Medicine: ‘The Low-Speed Concept’. In: Tatullo M. MSCs and Innovative Biomaterials in Dentistry. Cham: Springer Nature Switzerland AG.; 2017. p. 21-42.
Saeed MA, El-Rahman MA, Helal ME, Zaher AR, Grawish ME. Efficacy of human platelet rich fibrin exudate vs fetal bovine serum on proliferation and differentiation of dental pulp stem cells. Int J Stem Cells 2017; 10(1):38-47. https://doi.org/10.15283/ijsc16067
Abuarqouba D, Awidi A, Abuharfeil N. Comparison of osteo/odontogenic differentiation of human adult dental pulp stem cells and stem cells from apical papilla in the presence of platelet lysate. Arch Oral Biol 2015; 60(10):1545-53. https://doi.org/10.1016/j.archoralbio.2015.07.007
Bagdadi KE, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A, et al. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept). Eur J Trauma Emerg Surg 2019; 45(3):467-9. https://doi.org/10.1007/s00068-017-0785-7
Asri SR, Setiati HD, Asrianti D, Margono A, Usman M, Yulianto I. Optimum concentration of platelet-rich fibrin lysate for human dental pulp stem cells culture medium. J Int Dent Med Res 2019; 12(1):105-10.
Illmilda, Asrianti D, Margono A, Julianto I, Wardoyo MP. Advanced platelet rich fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation. J Int Dent Med Res 2019; 12(2):396-400.
Berridge MJ. Cell Cycle and Proliferation. Cell Signal Biol 2012; 1-42. https://doi.org/10.1042/csb0001009
Berridge MJ. Development. Cell Signal Biol 2012; 1-29. https://doi.org/10.1042/csb0001008
Bieback K. Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures. Transfus Med Hemother 2013; 40(5):326-35. https://doi.org/10.1159/000354061
Asrianti D, Margono A, Swastiningtyas S, Asri ISR, Usman M, Yulianto I. Comparison of human platelet lysate and fetal bovine serum in culture media for human dental pulp stem cell proliferation. Int J Appl Pharm 2019; 11:157-59. https://doi.org/10.22159/ijap.2019.v11s1.16025
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