Immunoglobulin G Levels in the Gingival Crevicular Fluid of Menopausal Patients with Periodontitis


  • Edmond Pradipta Andrianus
  • Sri Lelyati C. Masulili
  • Robert Lessang
  • Fatimah Maria Tadjoedin
  • Dimas Ilham Hutomo
  • Pitu Wulandari
  • Elza Ibrahim Auerkari


Immunoglobulin G, Periodontal Diseases, Periodontitis, Menopause


Objective: To measure the level of immunoglobulin G (IgG) in the gingival crevicular fluid (GCF). Material and Methods: A total of 158 patients aged >45 years were examined for periodontitis and interviewed regarding their menopausal status. The non-menopause group entailed female patients with periodontitis without menopause (n=23). The menopause group included females who stopped menstruating since >1 year, had a pocket depth of 4–5 mm, and did not have other systemic conditions (n=40). Samples were selected based on periodontal and menopausal status. In total, 63 samples of GCF were collected from the participants and tested using an enzyme-linked immunosorbent assay kit for IgG. Results: The median level of IgG in the menopause group was 39.50 g/mL, whereas that of the non-menopause group was 41.08 g/mL. There was a positive correlation between the plaque index and IgG level in both groups. In contrast, there was a negative correlation between age and IgG level. However, there was no correlation between plaque index and age regarding the IgG level in both groups (p>0.05). Conclusion: The IgG levels in the menopause group were lower than those in the non-menopause group. As such, menopausal females should take great care of their overall health, including the periodontium.


Haas AN, Rösing CK, Oppermann RV, Albandar JM, Susin C. Association among menopause, hormone replacement therapy, and periodontal attachment loss in Southern Brazilian women. J Periodontol 2009; 80(9):1380-7.

Gomes-Filho IS, Oliveira TJS, Passos JS, Cerqueira EMM, Cruz SS, Barreto ML, et al. Effect of osteoporosis on periodontal therapy among post-menopausal women. Gerodontology 2013; 30(1):40-8.

Pavičin IS, Dumančić J, Jukić T, Badel T. The relationship between periodontal disease, tooth loss and decreased skeletal bone mineral density in ageing women. Gerodontology 2017; 34(4):441-5.

Shi T, Min M, Sun C, Zhang Y, Liang M, Sun Y. Periodontal disease and susceptibility to breast cancer: A meta-analysis of observational studies. J Clin Periodontol 2018; 45(9):1025-33.

Mai X, Genco RJ, LaMonte MJ, Hovey KM, Freudenheim JL, Andrews CA, et al. Periodontal pathogens and risk of incident cancer in postmenopausal females: The Buffalo OsteoPerio Study. J Periodontol 2016; 87(3):257-67.

Taichman LS, Inglehart MR, Giannobile WV, Braun T, Kolenic G, Van Poznak CV. Periodontal health in women with early-stage postmenopausal breast cancer newly on aromatase inhibitors: a pilot study. J Periodontol 2015; 86(7):906-16.

Varghese T, Madhumala R, Ravi RS, Varghese A. Evaluation of periodontal status among premenopausal and postmenopausal women- a comparative study. Ann Int Med Dent Res 2016; 2(6):46-9.

Yoshida N, Sugimoto K, Suzuki S, Kudo H. Change in oral health status associated with menopause in Japanese dental hygienists. Int J Dent Hyg 2018; 16(1):157-64.

Khosravisamani M, Maliji G, Seyfi S, Azadmehr A, Nikfarjam BA, Madadi S, et al. Effect of the menstrual cycle on inflammatory cytokines in the periodontium. J Periodontal Res 2014; 49(6):770-6.

Chandra RV, Sailaja S, Reddy AA. Estimation of tissue and crevicular fluid oxidative stress marker in premenopausal, perimenopausal and postmenopausal women with chronic periodontitis. Gerodontology 2017; 34(3):382-9.

Scardina GA, Messina P. Oral microcirculation in post-menopause: A possible correlation with periodontitis. Gerodontology 2012; 29(2):1045-51.

Huynh AHS, Veith PD, McGregor NR, Adams GG, Chen D, Reynolds EC, et al. Gingival crevicular fluid proteomes in health, gingivitis and chronic periodontitis. J Periodontal Res. 2015; 50(5):637-49.

Marshall JS, Warrington R, Watson W, Kim HL. An introduction to immunology and immunopathology. Allergy Asthma Clin Immunol 2018; 14(Suppl 2):49.

Engdahl C, Bondt A, Harre U, Raufer J, Pfeifle R, Camponeschi A, et al. Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis : a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women. Arthritis Res Ther 2018; 20(1):84.

Alves RC, Félix SA, Rodriguez-Archilla A, Oliveira P, Brito J, Santos JM. Relationship between menopause and periodontal disease: a cross-sectional study in a Portuguese population. Int J Clin Exp Med 2015; 8(7):11412-9.

Choi Y, McKeown RE, Mayer-Davis EJ, Liese AD, Song K, Merchant AT. Serum C-reactive protein and immunoglobulin g antibodies to periodontal pathogens may be effect modifiers of periodontitis and hyperglycemia. J Periodontol. 2014; 85(9):1172-81.

Yilmazer M, Fenkci V, Fenkci S, Aktepe O, Sonmezer M, Kurtay G. Association of serum complement (C3, C4) and immunoglobulin (IgG, IgM) levels with hormone replacement therapy in healthy post-menopausal women. Hum Reprod. 2003; 18(7):1531-5.

Costalonga M, Herzberg MC. The oral microbiome and the immunobiology of periodontal disease and caries. Immunol Lett 2014; 162(2 Pt A):22-38.

Khan D, Ahmed SA. The immune system is a natural target for estrogen action: Opposing effects of estrogen in two prototypical autoimmune diseases. Front Immunol. 2016; 6:635.

Wulandari P, Masulili SLC, Tadjoedin FM, Kemal Y, Kusdhany LS. The relationship between estrogen levels and periodontal status in postmenopausal women. J Int Dent Med Res 2017;10(September Issue):657-62.




How to Cite

Andrianus, E. P. ., Masulili, S. L. C. ., Lessang, R. ., Tadjoedin, F. M. ., Hutomo, D. I. ., Wulandari, P. ., & Auerkari, E. I. . (2021). Immunoglobulin G Levels in the Gingival Crevicular Fluid of Menopausal Patients with Periodontitis. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 21, e5940. Retrieved from



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