Sucrose, Lactose, and Xylitol Exposures Affect Biofilm Formation of Streptococcus mutans


  • Prawati Nuraini
  • Indah Listiana Kriswandini
  • Rini Devijanti Ridwan
  • Soetjipto


Biofilms, Dental Plaque, Streptococcus mutans, Disaccharides, Sugar Alcohols


Objective: To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material and Methods: This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (α=0.05). Results: There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p<0.05). An LSD test indicated significant differences among the biofilm formations after exposure to 5% sucrose and 8% lactose and between 5% sucrose and 1% xylitol. In comparison, there were no significant differences (p>0.05) between 8% lactose and 1% xylitol. Conclusion: Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol.


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How to Cite

Nuraini, P. ., Kriswandini, I. L. ., Ridwan, R. D. ., & Soetjipto. (2021). Sucrose, Lactose, and Xylitol Exposures Affect Biofilm Formation of Streptococcus mutans. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 21, e0257. Retrieved from



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