Assessment of the Relationship Between Matrix Metalloproteinase-9 Promoter Gene Polymorphism and Chronic Periodontitis


  • Hiroj Bagde
  • Savitha Banakar
  • Alka Waghmare
  • Ashwini Bagde
  • Shailendra Singh Chaturvedi
  • Santosh Rayagouda Patil


Periodontitis, Genetics, Matrix Metalloproteinase 9


Objective: To evaluate the association of MMP-9 promoter gene polymorphism in generalized chronic periodontitis patients of an Indian population. Material and Methods: Eighty patients were selected and divided into case and control groups (n=40). Patients included were aged 15-60 years of both genders and diagnosed with chronic periodontitis. Peripheral venous blood samples were obtained, and genomic DNA was isolated by a 'salting out' method. Three possible genotypes were distinguished by three distinct banding patterns, depending on the presence or absence of the Sph I restriction site: CC as allele 1, TT as allele 2, and CT, respectively. Statistical analysis was carried out by applying the Chi-squared test and One-way ANOVA. Results: There was a significant difference in MMP-9 genotypes between Chronic periodontitis patients and healthy controls. Odds ratios for CT genotype and combination of CT and TT genotypes were 3.125 (95% confidence interval, p=0.028) and 3.667 (95% confidence interval, p=0.006) relative to subjects with CC genotype, respectively. Conclusion: The present study revealed an association of MMP-9 (-1562 C/T) gene polymorphism with generalised chronic periodontitis.


Könönen E, Gursoy M, Gursoy UK. Periodontitis: a multifaceted disease of tooth-supporting tissues. J Clin Med 2019; 8(8):1135.

Murakami S, Mealey BL, Mariotti A, Chapple ILC. Dental plaque-induced gingival conditions. J Periodontol 2018; 89(Suppl1):S17-S27.

Helmi MF, Huang H, Goodson JM, Hasturk H, Tavares M, Natto ZS. Prevalence of periodontitis and alveolar bone loss in a patient population at Harvard School of Dental Medicine. BMC Oral Health 2019; 19(1):254.

Loos BG, John RP, Laine ML. Identification of genetic risk factors for periodontitis and possible mechanisms of action. J Clin Periodontol 2005; 32(Suppl 6):159-79.

Mashhadiabbas F, Neamatzadeh H, Foroughi E, Dastgheib SA, Farahnak S, Nasiri R, et al. Association of MMP-2-753C>T and MMP-9-1562C>T polymorphisms with chronic/aggressive periodontitis risk: a systematic review and meta-analysis. Iran J Public Health 2019; 48(7):1227-38.

Aloka D, Padmakumar SK, Sathyan S, Sebastian M, Banerjee M, Beena VT. Association of matrix metalloproteinase 2 and matrix metalloproteinase 9 gene polymorphism in aggressive and nonaggressive odontogenic lesions: A pilot study. J Oral Maxillofac Pathol 2019; 23(1):158.

Alotaibi DH, Altalhi AM, Sambawa ZM, Koppolu P, Alsinaidi AA, Krishnan P. The association of matrix metalloproteinase gene polymorphisms and periodontitis: an overview. J Pharm Bioallied Sci 2020; 12(Suppl 1):S37-S42.

Michalowicz BS. Genetic and heritable risk factors in periodontal disease. J Periodontol 1994; 65(5 Suppl):479-88.

Hassell TM, Harris EL. Genetic influences in caries and periodontal diseases. Crit Rev Oral Biol Med 1995; 6(4):319-42.

Hodge P, Michalowicz B. Genetic predisposition to periodontitis in children and young adults. Periodontol 2000 2001; 26:113-34.

Kornman KS, Crane A, Wang HY, di Giovine FS, Newman MG, Pirk FW, et al. The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 1997; 24(1):72-7.

Malo D, Skamene E. Genetic control of host resistance to infection. Trends Genet 1994; 10(10):365-71.

Keles GC, Gunes S, Sumer AP, Sumer M, Kara N, Bagci H, et al. Association of matrix metalloproteinase-9 promoter gene polymorphism with chronic periodontitis. J Periodontol 2006; 77(9):1510-4.

Dyer JK, Peck MA, Reinhardt RA, Duckworth WC, Booth SJ, Seymour GJ, et al. HLA-D types and serum IgG responses to Capnocytophaga in diabetes and periodontitis. J Dent Res 1997; 76(12):1825-32.

Ryan ME, Golub LM. Modulation of matrix metalloproteinase activities in periodontitis as a treatment strategy. Periodontol 2000 2000; 24:226-38.

Souza JA, Rossa C Jr, Garlet GP, Nogueira AV, Cirelli JA. Modulation of host cell signaling pathways as a therapeutic approach in periodontal disease. J Appl Oral Sci 2012; 20(2):128-38.

Uitto VJ, Overall CM, McCulloch C. Proteolytic host cell enzymes in gingival crevice fluid. Periodontol 2000 2003; 31:77-104.

Pozo P, Valenzuela MA, Melej C, Zaldívar M, Puente J, Martínez B, et al. Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients. J Periodontal Res 2005; 40(3):199-207.

Birkedal-Hansen H. Role of matrix metalloproteinases in human periodontal diseases. J Periodontol 1993; 64(5 Suppl):474-84.

Ingman T, Tervahartiala T, Ding Y, Tschesche H, Haerian A, Kinane DF, et al. Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. J Clin Periodontol 1996; 23(12):1127-32.

Tervahartiala T, Pirila E, Ceponis A, Maisi P, Salo T, Tuter G, et al. The in vivo expression of the collagenolyticmatrix metalloproteinases (MMP-2, -8, -13, and -14) and matrilysin (MMP-7) in adult and localized juvenile periodontitis. J Dent Res 2000; 79(12):1969-77.

Ejeil AL, Igondjo-Tchen S, Ghomrasseni S, Pellat B, Godeau G, Gogly B. Expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in healthy and diseased human gingiva. J Periodontol 2003; 74(2):188-95.

Pan Y, Li D, Cai Q, Zhang W, Ma J, Wang M, et al. MMP-9 -1562C>T contributes to periodontitis susceptibility. J Clin Periodontol 2013; 40:125-30.

Löe H. The Gingival Index, the Plaque Index and the Retention Index Systems. J Periodontol 1967; 38(6):Suppl:610-6.

Theilade E, Wright WH, Jensen SB, Löe H. Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation. J Periodontal Res 1966; 1:1-13.

Kinane DF, Hart TC. Genes and gene polymorphisms associated with periodontal disease. Crit Rev Oral Biol Med 2003; 14(6):430-49.

Westerlund U, Ingman T, Lukinmaa PL, Salo T, Kjeldsen L, Borregaard N, et al. Human neutrophil gelatinase and associated lipocalin in adult and localized juvenile periodontitis. J Dent Res 1996; 75(8):1553-63.

de Souza AP, Trevilatto PC, Scarel-Caminaga RM, de Brito RB Jr, Barros SP, Line SR. Analysis of the MMP-9 (C-1562 T) and TIMP-2 (G-418C) gene promoter polymorphisms in patients with chronic periodontitis. J Clin Periodontol 2005; 32(2):207-11.

Holla LI, Fassmann A, Muzík J, Vanek J, Vasku A. Functional polymorphisms in the matrix metalloproteinase-9 gene in relation to severity of chronic periodontitis. J Periodontol 2006; 77(11):1850-5.

Gürkan A, Emingil G, Saygan BH, Atilla G, Çınarcık S, Köse T, et al. Matrix metalloproteinase-2, -9, and -12 gene polymorphisms in generalized aggressive periodontitis. J Periodontol 2007; 78(12):2338-47.




How to Cite

Bagde, H. ., Banakar, S. ., Waghmare, A. ., Bagde, A., Chaturvedi, S. S. ., & Patil, S. R. . (2021). Assessment of the Relationship Between Matrix Metalloproteinase-9 Promoter Gene Polymorphism and Chronic Periodontitis. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 21, e0264. Retrieved from



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