Silver Diamine Fluoride versus Bioactive Giomer Light-Curing Varnish: An In Vitro Study on Caries Arrest

Maressa Borges  dos Reis; Monique Gonçalves D' Alessandro; Kenia Aparecida Freitas  Moraes; Suéllen Peixoto de Medeiros  Urquiza; Bruna Lorena Pereira  Moro; Tamara Kerber  Tedesco; José Carlos Pettorossi  Imparato

Authors

Maressa Borges dos Reis
Monique Gonçalves D' Alessandro
Kenia Aparecida Freitas Moraes
Suéllen Peixoto de Medeiros Urquiza
Bruna Lorena Pereira Moro
Tamara Kerber Tedesco
José Carlos Pettorossi Imparato

Keywords:

Dental Caries, Fluorides, Mechanical Tests, Hardness Tests, Dentin

Abstract

Objective: To compare the capacity of silver diamine fluoride (30%) and the bioactive giomer light-curing varnish for arresting ICDAS score 5 non-retentive caries lesions. Material and Methods: An in vitro analytical study was conducted using 36 healthy primary teeth, in which a cavity was created, divided into 3 groups: control (distilled and deionized water), giomer-based light-curing varnish - Barrier Coat, and Silver Diamine Fluoride 30% – Cariestop (SDF). The samples were analyzed for mineral loss as measured by surface microhardness (SMH). The Knoop microhardness test was performed using the HVS-1000 microhardness tester. The data were compared by two-way analysis of variance – treatment and time (before and after treatment) followed by Tukey's post-test. The significance level adopted in all analyzes was 5%. Results: The specimens treated with giomer showed higher SMH compared to the untreated group but were similar to those of the SDF group. The SDF group, on the other hand, showed no statistically significant difference from the control group (p=0.010). Conclusion: The giomer varnish had a remineralizing effect on artificial dentinal caries.

References

Mohammadi N, Farahmand Far MH. Effect of fluoridated varnish and silver diamine fluoride on enamel demineralization resistance in primary dentition. J Indian Soc Pedod Prev Dent 2018; 36(3):257-61. https://doi.org/10.4103/JISPPD.JISPPD_4_18

Gao SS, Zhao IS, Hiraishi N, Duangthip D, Mei ML, Lo ECM, et al. Clinical trials of silver diamine fluoride in arresting caries among children: a systematic review. JDR Clin Trans Res 2016; 1(3):201-10. https://doi.org/10.1177/2380084416661474

Burgess JO, Vaghela PM. Silver diamine fluoride: a successful anticarious solution with limits. Adv Dent Res 2018; 29(1):131-4. https://doi.org/10.1177/0022034517740123

Fung MHT, Duangthip D, Wong MCM, Lo ECM, Chu CH. Randomized clinical trial of 12% and 38% silver diamine fluoride treatment. J Dent Res 2018; 97(2):171-8. https://doi.org/10.1177/0022034517728496

Hu S, Meyer B, Duggal M. A silver renaissance in dentistry. Eur Arch Paediatr Dent 2018; 19(4):221-7. https://doi.org/10.1007/s40368-018-0363-7

Alsayed EZ, Hariri I, Nakashima S, Shimada Y, Bakhsh TA, Tagami J, et al. Effects of coating materials on nanoindentation hardness of enamel and adjacent areas. Dent Mater 2016; 32(6):807-16. https://doi.org/10.1016/j.dental.2016.03.023

Rabiabasree R, Krishnakumar R, Prabhu AS, Naik NS, Shashibhushan KK, Janarthanan K. Inhibitory effect of a resin coat-containing prereacted glass fillers on the enamel demineralization of the primary teeth: An in vitro pilot study. J Indian Soc Pedod Prev Dent 2019; 37(2):146-50. https://doi.org/10.4103/JISPPD.JISPPD_309_18

Arita S, Suzuki M, Kazama-Koide M, Shinkai K. Shear bond strengths of tooth coating materials including the experimental materials contained various amounts of multi-ion releasing fillers and their effects for preventing dentin demineralization. Odontology 2017; 105(4):426-36. https://doi.org/10.1007/s10266-016-0290-1

Ma S, Imazato S, Chen JH, Mayanagi G, Takahashi N, Ishimoto T, et al. Effects of a coating resin containing S-PRG filler to prevent demineralization of root surfaces. Dent Mater J 2012; 31(6):909-15. https://doi.org/10.4012/dmj.2012-061

Kotaku M, Murayama R, Shimamura Y, Takahashi F, Suzuki T, Kurokawa H, et al. Evaluation of the effects of fluoride-releasing varnish on dentin demineralization using optical coherence tomography. Dent Mater J 2014; 33(5):648-55. https://doi.org/10.4012/dmj.2014-072

Delbem AC, Bergamaschi M, Sassaki KT, Cunha RF. Effect of fluoridated varnish and silver diamine fluoride solution on enamel demineralization: pH-cycling study. J Appl Oral Sci 2006; 14(2):88-92. https://doi.org/10.1590/s1678-77572006000200005

Marquezan M, Corrêa FN, Sanabe ME, Rodrigues Filho LE, Hebling J, Guedes-Pinto AC, et al. Artificial methods of dentine caries induction: A hardness and morphological comparative study. Arch Oral Biol 2009; 54(12):1111-7. https://doi.org/10.1016/j.archoralbio.2009.09.007

Vale GC, Tabchoury CP, Del Bel Cury AA, Tenuta LM, ten Cate JM, Cury JA. APF and dentifrice effect on root dentin demineralization and biofilm. J Dent Res 2011; 90(1):77-81. https://doi.org/10.1177/0022034510383428

Chu CH, Lo EC. Promoting caries arrest in children with silver diamine fluoride: a review. Oral Health Prev Dent 2008; 6(4):315-21.

Mei ML, Lo ECM, Chu CH. Arresting dentine caries with silver diamine fluoride: what's behind it? J Dent Res 2018; 97(7):751-8. https://doi.org/10.1177/0022034518774783

Zhao IS, Yin IX, Mei ML, Lo ECM, Tang J, Li Q, et al. Remineralising dentine caries using sodium fluoride with silver nanoparticles: an in vitro study. Int J Nanomed 2020; 15:2829-39. https://doi.org/10.2147/IJN.S247550

Zhao IS, Gao SS, Hiraishi N, Burrow MF, Duangthip D, Mei ML, et al. Mechanisms of silver diamine fluoride on arresting caries: a literature review. Int Dent J 2018; 68(2):67-76. https://doi.org/10.1111/idj.12320

Gao SS, Chen KJ, Duangthip D, Wong MCM, Lo ECM, Chu CH. Preventing early childhood caries with silver diamine fluoride: study protocol for a randomised clinical trial. Trials 2020; 21(1):140. https://doi.org/10.1186/s13063-020-4088-7

Ando T. Indicações do uso do diaminofluoreto de prata (Saforide) em odontopediatria. RGO 1981; 29(1):48-9. [In Portuguese].

Nishino M, Massler M. Immunization of caries susceptible pits and fissures with a diamine silver fluoride solution. J Pedod 1977; 2(1):16-25.

Lansdown AB. Silver. I: Its antibacterial properties and mechanism of action. J Wound Care 2002; 11(4):125-30. https://doi.org/10.12968/jowc.2002.11.4.26389

Lansdown AB. Silver in health care: antimicrobial effects and safety in use. Curr Probl Dermatol 2006; 33:17-34. https://doi.org/10.1159/000093928

Wu MY, Suryanarayanan K, van Ooij WJ, Oerther DB. Using microbial genomics to evaluate the effectiveness of silver to prevent biofilm formation. Water Sci Technol 2007; 55(8-9):413-9. https://doi.org/10.2166/wst.2007.285

Knight GM, McIntyre JM, Craig GG, Mulyani. Ion uptake into demineralized dentine from glass ionomer cement following pretreatment with silver fluoride and potassium iodide. Aust Dent J 2006; 51(3):237-41. https://doi.org/10.1111/j.1834-7819.2006.tb00435.x

Zhao IS, Mei ML, Burrow MF, Lo EC, Chu CH. Prevention of secondary caries using silver diamine fluoride treatment and casein phosphopeptide-amorphous calcium phosphate modified glass-ionomer cement. J Dent 2017; (57):38-44. https://doi.org/10.1016/j.jdent.2016.12.001

Wambier DS, Bosco VL. The use of cariostatic agents in pediatric dentistry. Rev Odontopediatr 1995; 4(1):35-41.

Köche JC. Fundamentos de Metodologia Científica: Teoria da Ciência e Prática da Pesquisa. 19. ed. Porto Alegre: Vozes; 2001. [In Portuguese].

de Almeida LF, Cavalcanti YW, Valença A. In vitro antibacterial activity of silver diamine fluoride in different concentrations. Acta Odontol Latinoam 2011; 24(2):127-31.

Igarashi S. Antibacterial Effect of Ag(NH3)2F solution on Streptococcus mutans in plaque. Jpn J Paediatr Dent 1978; (1):1-18.